Pro35SLBD16:GR or Pro35SLBD18:GR transgenic seedlings that overexpress LBD16 or LBD18 fused to glucocorticoidsteroid hormone binding domain(GR) under CaMV35S promoter were grown for 12 days under long-day conditions (16h light/ 8h dark).

Transcription profiling by array of Arabidopsis overexpressing LBD16 and LBD18 to investigate downstream genes

LBD18 acts as a transcriptional activator that directly binds to the EXPANSIN14 promoter in promoting lateral root emergence of Arabidopsis

Affymetrix GeneChip Arabidopsis Genome [ATH1-121501]

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Pro35SLBD16:GR or Pro35SLBD18:GR transgenic seedlings that overexpress LBD16 or LBD18 fused to glucocorticoidsteroid hormone binding domain(GR) under CaMV35S promoter were grown for 12 days under long-day conditions (16h light/ 8h dark).


Affymetrix:Protocol:ExpressionStat

Title: Affymetrix CHP Analysis (ExpressionStat). Description:

Affymetrix:Protocol:Hybridization-FS450_0004[]

P-AFFY-2

Labeling of samples was carried out according to the manufacturer’s standard protocol

P-AFFY-6

Title: Affymetrix CEL analysis. Description:

Pro35SLBD16:GR or Pro35SLBD18:GR transgenic seedlings that overexpress LBD16 or LBD18 fused to glucocorticoidsteroid hormone binding domain(GR) under CaMV35S promoter were grown for 12 days under long-day conditions (16h light/ 8h dark) on the Murashige Skoog(MS) medium containing vitamins, 1.5% sucrose, 2.5 mM Mes, pH 5.7, and 0.8% agar, treated with 10 uM dexamethasone (DEX) or mock-treated for 2.5 h.

Pro35SLBD16:GR or Pro35SLBD18:GR transgenic seedlings that overexpress LBD16 or LBD18 fused to glucocorticoidsteroid hormone binding domain(GR) under CaMV35S promoter were grown for 12 days under long-day conditions (16h light/ 8h dark) on the ½ Murashige Skoog(MS) medium containing vitamins, 1.5% sucrose, 2.5 mM Mes, pH 5.7, and 0.8% agar, treated with 10 uM dexamethasone (DEX) or mock-treated for 2.5 h.

Equal amounts of RNA from triplicate samples were pooled.

Each RNA sample is obtained from seedlings of Arabidopsis. The frozen plant tissue (~100 mg) was ground in tiny mortars dipped in liquid nitrogen. The frozen powder was carefully transfered into 1.5 ml microcentrifuge tubes using small funnels and rubber spatulas also cooled with liquid nitrogen. Total RNA was extracted using RNeasy Mini kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer's instructions and purified with an RNeasy minelute cleanup (Qiagen).

Transcription profiling by array of Arabidopsis overexpressing LBD16 and LBD18 to investigate downstream genes

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