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Accession IconE-MEXP-2064

Transcription profiling of Drosophila after exposure to microgravity in the International Space Station and in a microgravity simulator

Organism Icon Drosophila melanogaster
Sample Icon 21 Downloadable Samples
Technology Badge Icon Affymetrix Drosophila Genome Array (drosgenome1)

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Description
Larvae-Pupae transition flies (Drosophila) were recovered and transport for 3 days at 12-14ºC to arrest development until the launch site, then exposed to RT (18-20ºC) for some hours including the launch and trip to the International Space Station, then pupae were exposed to microgravity in the ISS for 4 days and a half at 22ºC. Finally pupae were fixed on acetone and frozen until recovery on Earth.<br></br><br></br><br></br><br></br>Four groups of samples: 1 ISS (+ground control) as described, 2 RPM (microgravity simulator on Earth) as described, 3 RPM without constrains (No MAMBA container and only 5 days exposure without cold transport) and 4 centrifuge 10g without constrains control..
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