Affymetrix GeneChip Human Genome HG-U133A [HG-U133A]

Conserved transcription factor binding sites of cancer markers derived from primary lung adenocarcinoma microarrays

Identification and characterisation of human apoptosis inducing proteins using cell-based transfection microarrays and expression analysis

Cells lacking IKKα show nuclear cyclin D1 overexpression and a neoplastic phenotype: role of IKKα as a tumor suppressor.

rawData

scan

Gene transcription in a set of 49 human primary lung adenocarcinomas and 9 normal lung tissue samples was examined using Affymetrix GeneChip technology. We aimed to investigate differential gene expression between the two tissue types. A total of 3,442 genes, called the set MAD, were found to be either up- or down-regulated by at least two fold between the two phenotypes. Genes assigned to a particular gene ontology term were found, in many cases, to be significantly unevenly distributed between the genes in and outside MAD. Terms that were overrepresented in MAD included functions directly implicated in cancer cell metabolism. Based on their functional roles and expression profiles, genes in MAD were grouped into likely co-regulated gene sets.

disease

Transcription profiling by array of human primary lung adenocarcinomas

"Gene transcription in a set of 49 human primary lung adenocarcinomas and 9 normal lung tissue samples was examined using Affymetrix GeneChip technology. We aimed to investigate differential gene expression between the two tissue types. A total of 3,442 genes, called the set MAD, were found to be either up- or down-regulated by at least two fold between the two phenotypes. Genes assigned to a particular gene ontology term were found, in many cases, to be significantly unevenly distributed between the genes in and outside MAD. Terms that were overrepresented in MAD included functions directly implicated in cancer cell metabolism. Based on their functional roles and expression profiles, genes in MAD were grouped into likely co-regulated gene sets."

Normal Lung + Lung adenocarcinoma microarray

Gene transcription in a set of 49 human primary lung adenocarcinomas and 9 normal lung tissue samples was examined using Affymetrix GeneChip technology. We aimed to investigate differential gene expression between the two tissue types. A total of 3,442 genes, called the set MAD, were found to be either up- or down-regulated by at least two fold between the two phenotypes. Genes assigned to a particular gene ontology term were found, in many cases, to be significantly unevenly distributed between the genes in and outside MAD. Terms that were overrepresented in MAD included functions directly implicated in cancer cell metabolism. Based on their functional roles and expression profiles, genes in MAD were grouped into likely co-regulated gene sets.


Affymetrix:Protocol:Hybridization-EukGE-WS2v4[]

Title: Affymetrix EukGE-WS2v4 Hybridization. Description:

P-AFFY-6

Title: Affymetrix CEL analysis. Description:

P-MEXP-6629

The RNA samples were processed as recommended by Affymetrix (Santa Clara, California). Briefly, total RNA was isolated from frozen cell pellets following using the TRIzol RNA isolation method (Life Technologies, Gaithersburg, Maryland). Reverse transcription was performed on 10 ug of total RNA with the use of SuperScript II polymerase (Life Technologies, Gaithersburg, Maryland) and an oligo-(dT)24 primer with a T7 RNA polymerase promoter (Genset, La Jolla, California). An RNA Transcript Labeling Kit (Enzo Diagnostics, Farmingdale, NY) was used for the production of hybridizable biotin-labeled cRNA targets by in vitro transcription from T7 RNA polymerase promoters, purified by Qiagen RNeasy Mini Kit (Qiagen, Chatsworth, California) and fragmented to sizes from 50 to 200 bases by incubation at 94 C for 35 min.

Transcription profiling by array of human primary lung adenocarcinomas

Submitter Supplied Information

Samples