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Accession IconE-MEXP-3519

RIP-chip by array of RNA-binding protein HuR in mouse early peripheral nerves

Organism Icon Mus musculus
Sample Icon 20 Downloadable Samples
Technology Badge IconIllumina_MouseWG-6_v2.0

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Description
Identify mRNA targets of the RNA-binding protein HuR in vivo during Schwann cell development using RIP-chip analysis. IP protocol of endogenous mRNA-transfected HuR complexes was performed as described in Keene et al. (2006). In brief, 500 mg of whole-cell lysate obtained from a pool of NB or P5 sciatic nerves from C57BL6J mice were incubated with a suspension of Protein Sepharose beads (Sigma-Aldrich), pre-coated with 15 mg of either IgG1 (BD Pharmingen) or anti-HuR (Santa Cruz Biotechnology) antibodies. mRNAs were isolated using the phenol-chloroform method.
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