Affymetrix GeneChip Human Genome HG-U133A [HG-U133A]

Abnormal muscle and hematopoietic gene expression may be important for clinical morbidity in primary hyperparathyroidism

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We analyzed mRNAs in transiliacal bone biopsies from 7 patients with primary hyperparathyroidism using Affymetrix HG-U133A Gene Chips Similar analyses of the global transcriptional activity were repeated in a second bone biopsy from the same patient taken one year after surgery and reversal of disease parameters.

disease

Transcription profiling by array of human transiliac bone biopsies from pre and post surgery to investigate the molecular pathology of bone in hyperparathyroidism

Transcription profiling of human transiliacal bone biopsies from 7 patients with primary hyperparathyroidism pre and post surgery to investigate the molecular pathology of bone in hyperparathyroidism

We analyzed mRNAs in transiliacal bone biopsies from 7 patients with primary hyperparathyroidism using Affymetrix HG-U133A Gene Chips Similar analyses of the global transcriptional activity were repeated in a second bone biopsy from the same patient taken one year after surgery and reversal of disease parameters.


Affymetrix:Protocol:ExpressionStat

Title: Affymetrix CHP Analysis (ExpressionStat). Description:

Affymetrix:Protocol:Hybridization-Unknown

P-AFFY-6

Title: Affymetrix CEL analysis. Description:

All patients had one bone biopsy taken before and one year after surgery (of parathyroid glands) from opposite but symmetrical places of os ileum to avoid woven bone. As local anesthetics could influence the cytokine responses all biopsies were taken with the same amount of local anesthesia (Lidocain). The biopsies were taken with Bordier's trephine and immediately frozen in liquid nitrogen and stored at -70 C for later RNA extraction

The frozen bone cylinder biopsies were pulverized with mortar in liquid nitrogen with their content of marrow intact. The pulverized bone was added to TRIZOL reagent (Life Technologies, Gaithersburg, MD), homogenized and RNA purified according to the manufacturers instructions. Routinely 50 - 80 ug total RNA was obtained and the quality controlled by gel electrophoresis. RNA was further purified prior to cDNA synthesis using the RNeasy kit (Qiagen) to remove organic components

Double stranded cDNA and biotin labeled cRNA probes were made from 5 ug total RNA using the Superscript Choice system (Invitrogen) and the Enzo Bioarray respectively. Procedures were performed according to recommendations from Affymetrix.

Transcription profiling by array of human transiliac bone biopsies from pre and post surgery to investigate the molecular pathology of bone in hyperparathyroidism

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