Affymetrix GeneChip Murine Genome U74A [MG_U74A]

Affymetrix GeneChip Murine Genome U74Av2 [MG_U74Av2]

Affymetrix GeneChip Human Genome U95A [HG_U95A]

The Utility of DNA Microarrays for Characterizing Genotoxicity

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Mouse lymphoma L5178Y and Human TK6 cells are treated with Methylmethane Sulfonate [CAS:66-27-3;CHEBI:25255] and harvested a 4 and 24 hours for analysis.

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Transcription profiling of mouse lymphoma L5178Y and Human TK6 cells treated with Methylmethane Sulfonate ILSI-HESI Genotoxicity Study - MMS

Mouse lymphoma L5178Y and Human TK6 cells are treated with Methylmethane Sulfonate [CAS:66-27-3;CHEBI:25255] and harvested a 4 and 24 hours for analysis.


P-TOXM-190

Mouse lymphoma L5178Y TK+/- cells were maintained in RPMI 1640 media supplemented with 2 mg/mL sodium bicarbonate, 10ug/mL folic acid, 1.5 mg/mL L-glutamine, 0.11 mg/mL sodium pyruvate, 0.5 mg/mL Pluronit F68 and 100 U/mL final concentration each of a pen/strep mixture all adjusted to a pH of 7.2.  Media was supplemented with 10% heat-inactivated horse serum for cell growth.

P-TOXM-191

Mouse TK6 cells were maintained in RPMI 1640 media supplemented with 2 mg/mL sodium bicarbonate, 10ug/mL folic acid and 100 U/mL final concentration each of a pen/strep mixture all adjusted to a pH of 7.2.  

P-TOXM-193

100mL of a 5 x 10^5 cells/mL culture is exposed to a defined concentration of each test chemical (dissolved in DMSO for final concentration of <= 1%) and incubated for 4 hours at 37 degrees C.

P-TOXM-194

Cells are washed twice with growth media through centrifugation for 5 min at 1200xg and resuspension.  Pellets are rinsed twice in ice-cold Dulbecco's PBS and finally subjected to TRIzol extraction protocol.  Samples are stored in DEPC treated water.

Transcription profiling of mouse lymphoma L5178Y and Human TK6 cells treated with Methylmethane Sulfonate ILSI-HESI Genotoxicity Study - MMS

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