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Accession IconGSE16108

Transcription profiling of parental lines and bulked salt sensitive and salt tolerant RILs derived from 2 rice varieties

Organism Icon Oryza sativa indica group
Sample Icon 16 Downloadable Samples
Technology Badge Icon Affymetrix Rice Genome Array (rice)

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Description
The aim of this study was to minimize the number of candidate genes responsible for salt tolerance between a pair of rice varieties (CSR27 and MI48) with contrasting level of salt tolerance by bulked segregant analysis of their recombinant inbred lines. Microarray analysis of RNA extracted from the tolerant and susceptible parents without and with stress showed 798 and 2407 differentially expressed genes, respectively. The number of differentially expressed genes was drastically reduced to 70 and 30, by pooling the RNAs from ten extreme tolerant and ten extreme susceptible RILs due to normalization of irrelevant differentially expressed genes between the parents.
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