The effect of cardiac troponin T2 knockdown on gene expression in zebrafish embryos

We examined the transcriptional effect of preventing cardiac contraction in zebrafish embryos which can be deprived of circulation without experiencing hypoxia since the fish obtain sufficient oxygen via diffusion. Morpholino antisense knockdown of cardiac troponin T2 (tnnt2) prevented cardiac contraction without affecting vascular development. We concluded that absence of hemodynamic force induces endothelial CXCR4a up-regulation and promotes recovery of blood flow.

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Packham IM, Gray C, Heath PR, Hellewell PG, Ingham PW, Crossman DC, Milo M, Chico TJ