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Accession IconGSE20231

Transcript profiling of auxin/cytokinin crosstalk in the Arabidopsis primary root apex

Organism Icon Arabidopsis thaliana
Sample Icon 14 Downloadable Samples
Technology Badge Icon Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)

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Description
AtIPT8/pga22 seedlings (gain-of-function mutant in Ws background; Sun et al. 2003, Plant Physiology 131, pp167-176) were grown on vertical plates for 7 days in LD. The seedlings were then incubated directly on the plate with medium containing 5 uM 17-beta-estradiol (for induction of the IPT8 gene) or 5 uM trans-zeatin for 12 and 24 h. 5 mm primary root tips were harvested from the seedlings and pooled for microarray analysis. Total RNA was isolated from the samples with the RNeasy Plant Mini Kit from Qiagen.
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