Real-time Monitoring of Cisplatin Toxicity on Cancer Cells

Treatment of MCF7 breast cancer cells by cisplatin leads to a very specific metabolic response and an onset of cell death about 10-11 h after beginning of treatment. For more detailed understanding of the molecular processes underlying the specific metabolic response, mRNA was isolated from MCF7 cells when the specific changes, (i) induction of glycolysis and (ii) onset of cell death, were detected during online measurement in the cell biosensor system.

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Alborzinia H, Can S, Holenya P, Scholl C, Lederer E, Kitanovic I, Wölfl S