Study of Mid-Blastula Transition of zebrafish embryos

Sox31 is a member of the zebrafish SoxB1 subfamily, and its expression can be detected both pre- and post-MBT. To distinguish the function of its maternal and zygotic transcripts, a splice blocking morpholino (Sb MO) was designed to interfere with the processing of new, zygotically synthesised mRNAs without interfering with mRNAs of maternal origin. Developmental arrest was observed in Sb MO which could not bypass MBT.

10

Hu SN, Yu H, Zhang YB, Wu ZL, Yan YC, Li YX, Li YY, Li YP