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Accession IconGSE57751

Sugar-dependent gene expression in xylose grown A. thaliana cell suspension culture

Organism Icon Arabidopsis thaliana
Sample Icon 12 Downloadable Samples
Technology Badge Icon Affymetrix Arabidopsis ATH1 Genome Array (ath1121501)

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Description
Sugars modulate expression of hundreds of genes in plants. Previous studies on sugar signaling, using intact plants or plant tissues, were hampered by tissue heterogeneity, uneven sugar transport and/or inter-conversions of the applied sugars. This, in turn, could obscure the identity of a specific sugar that acts as a signal affecting expression of given gene in a given tissue or cell-type. To bypass those biases, we have developed a novel biological system, based on stem-cell-like Arabidopsis suspension culture. The cells were grown in a hormone-free medium and were sustained on xylose as the only carbon source. The functional genomics approach was used to identify sugar responsive genes, which rapidly (within 1 h) respond specifically to low concentration (1 mM) of glucose, fructose and/or sucrose.
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