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Accession IconGSE98793

Gene expression analysis in whole blood samples obtained from donors diagnosed with MDD compared to healthy controls

Organism Icon Homo sapiens
Sample Icon 188 Downloadable Samples
Technology Badge Icon Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2)

Submitter Supplied Information

Description
This study investigated gene expression changes in whole blood samples obtained from donors diagnosed with major depressive disorder (MDD) compared to healthy controls. Micro-array data were available from whole blood on patients with MDD (N=128, 64 with generalised anxiety disorder, diagnosed by the MINI questionnaire, and 64 without anxiety disorder) and healthy controls (N=64). RNA was isolated from all samples using the standard PAXgene protocol on the Qiagen Biorobot 8000. All samples gave good quality RNA, as assessed by Agilent Bioanalyser. The yield range was 0.86-15.05ug with an average of 6.25ug. Samples were then randomised into batches, with each batch containing a representative number of controls, depression with anxiety and depression without anxiety, and the same ratio of females to males (3:1). 50ng of RNA from each sample was converted to a biotin labeled cDNA probe using NuGEN SPIA amplification. The probes were then hybridized to Affymetrix U133_Plus2.0 Genechips.
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Total Samples
192
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Samples

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whole_blood_control_5
male
53.400
whole blood
whole_blood_control_27
female
50.900
whole blood
whole_blood_case_7
male
57.800
whole blood
whole_blood_control_10
female
69.600
whole blood
whole_blood_control_8
female
64.500
whole blood
whole_blood_case_60
female
72.200
whole blood
whole_blood_case_53
female
64.700
whole blood
whole_blood_case_81
male
35.900
whole blood
whole_blood_case_108
female
67.100
whole blood
whole_blood_case_89
male
55.400
whole blood
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