Half-life determination for human B-cells (BL41)

RNA was labeled in BL41 cells by culturing cells for 60 min in media containing 100M 4sU. Tc-RNA was separated into nt- and p-RNA. All three RNA subsets were subjected to microarray analysis. Only probe sets providing present calls in all RNA samples/subsets were included into the analysis

9

Friedel CC, Dölken L, Ruzsics Z, Koszinowski UH, Zimmer R