github link
Accession IconSRP008774

Small RNA profiling in Drosophila wild-type and nbr[f02257] mutants

Organism Icon Drosophila melanogaster
Sample Icon 2 Downloadable Samples
Technology Badge IconIllumina Genome Analyzer

Submitter Supplied Information

Description
nbr/CG9247 gene regulates 3''end heterogeneity of a subset of miRNAs. It is not clear how broad this effect is on small RNA population. To address this, we compared small RNA population in wild-type and tmr[f02257] mutants. This approach identified more miRNAs whose 3''end heterogeneity was affected in nbr[f02257] mutants. Overall design: 2-3 day old control (w homogeneous strain Bloomington stock center 5905) and nbr[f02257] null mutant flies were collected. nbr[f02257] line was in the homogenous (Bloomington stock center stock 5905) background through a minimum of 5 backcrosses. Total RNA from whole flies was extracted using TRIzol reagent (Invitrogen). 40ug of total RNA from each genotype was used for small RNA library preparation with Small RNA Sample Prep kit (v1.5) (Illumina).
PubMed ID
Total Samples
2
Submitter’s Institution
No associated institution

Samples

Show of 0 Total Samples
Filter
Add/Remove
Accession Code
Title
Sex
Specimen part
Subject
Processing Information
Additional Metadata
No rows found
Loading...