Illumina HiSeq 2500 sequencing; GSM1215320: endosperm_12DAP_r1; Zea mays; RNA-Seq

endosperm_12DAP_r1

An atlas of RNA and protein expression maps across a diverse set of developmental tissues from Zea mays Overall design: RNA-seq collected from a variety of maize tissues

Maize proteome and transcriptome atlas

Submitted by Gene Expression Omnibus on 19-AUG-2016

RNA-seq libraries were prepared as follows. Approximately four micrograms of total RNA was used as input. Each sequencing library was constructed using the TruSeq RNA Sample Prep Kit V2 (Illumina, San Diego, CA) according to manufacturerâ€™s instructions with the following modifications to confer strand-specificity. The polyA-selected RNA was used for first strand synthesis and the resulting cDNA:RNA hybrid was purified using RNAClean XP beads (Beckman, Brea, CA). The second strand synthesis was performed using a dNTP mix containing dUTPs (10mM dATPs, 10mM dGTPs, 10mM dCTPs, and 20mM dUTPs) and DNA Polymerase I (New England Biolabs, Ipswich, MA). The purified ligation products were incubated with Uracil DNA Glycosylase (Fermentas) before PCR amplification.

Maize proteome and transcriptome atlas

Submitter Supplied Information

Samples