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Accession IconSRP039957

Transcriptional response to stress in serum deprived mouse fibroblasts [RNA-Seq]

Organism Icon Mus musculus
Sample Icon 4 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

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Description
We have employed gene expression profiling in order to identify targets of transcriptional response to stress in resting mouse Swiss 3T3 fibroblasts, either untreated (control) or treated with anisomycin to induce the p38/MAP kinase pathway. Overall design: Serum starved (72 h 0.2% FCS) mouse 3T3 cells were treated with anisomycin (188.5 nM) for 1 h (in duplicates). Untreated, serum-starved cells were used as a control. RNA was collected and gene expression profiling using strand-specific RNA-seq was performed.
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4
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