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Accession IconSRP056200

Ribo_seq (aka ribosome profiling) analysis of control and Myc-induced U2OS cells

Organism Icon Homo sapiens
Sample Icon 10 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

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Description
We used Ribo-seq to examine the effect of Myc activation on protein translation in U2OS cells and correalted these changes with alterations in RNA level measured by RNA-seq on tye same conditions. We also examined these effects in the presence of Torin-1, an inhibitor of mTOR Overall design: We measure ribosome occupancy profiles in U2OS cells containing an inducible Myc expression vector that were induced or mock-treated in duplicates for 36 hours. In addition, we repeated the experiments in the presence of Torin-1, an inhibitor of mTOR.
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10
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