Ribosomal footprinting of CN34-Parental and CN34-LM1a

We used Illlumina Artseq Ribo Profile to perform ribosome footprinting on the above cell lines Overall design: We followed the manufacturer's protocol: Cells were treated with cycloheximide (0.1 mg/ml) to inhibit translation; total RNA(tt) and ribosome-protected RNA fragments (RPF) were isolated and library preped for sequencing simultaneously.

8

Goodarzi H, Nguyen HCB, Zhang S, Dill BD, Molina H, Tavazoie SF