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Accession IconSRP069011

Ribosomal footprinting of MDA_Ctrl and MDA_Glu overexpression cell lines

Organism Icon Homo sapiens
Sample Icon 24 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000, NextSeq 500

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Description
We used Illlumina Artseq Ribo Profile to perform ribosome footprinting on the above cell lines Overall design: We followed the manufacturer's protocol: Cells were treated with cycloheximide (0.1 mg/ml) to inhibit translation; total RNA and ribosome-protected RNA fragments were isolated and library preped for sequencing simultaneously.
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24
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