Illumina HiSeq 2500 sequencing; GSM2248122: Control_D0; Homo sapiens; RNA-Seq

Control_D0

We use time series RNA-seq to conduct a genome-wide survey of the temporal transcriptome response of human embryonic stem (ES) cell-derived neural progenitor cells (NPCs) exposed to lead. Overall design: NPCs were derived from human embryonic stem cells (hESCs) with a modified protocol from a previously reported protocol (Chambers et al. 2009) (Methods). We used lead acetate to treat NPCs at two different concentrations, 3 µM and 30 µM.

RNA-seq of Human neural progenitor cells exposed to lead (Pb) reveals transcriptome dynamics, splicing alterations and Pb disease risk associations

Submitted on 02-JAN-2018

Samples were collected at indicated time points by lysing cells directly on the plate with 350ul RLT buffer. Cells were lysed in 350ul of the Qiagen Buffer RLT (79216). Total RNA was isolated using the Qiagen RNeasy 96 Kit (74181) and quantified with the ThermoFisher Scientific Quant-It RNA Assay Kit (Q33140) and the Agilent Bioanalyzer RNA 6000 Pico Kit (5067-1513). cDNA libraries were prepared with 100 ng of input total RNA and indexed with the Illumina TruSeq RNA Prep Kit v2 (RS-122-2001 and RS-122-2002). Indexed cDNA libraries were pooled and sequenced on an Illumina HiSeq2500 with single end 51 bp reads.

RNA-seq of Human neural progenitor cells exposed to lead (Pb) reveals transcriptome dynamics, splicing alterations and Pb disease risk associations

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