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Accession IconSRP096545

Comparison of translational profiles in Motor Neurons (CHAT), to all neurons (Snap25) in the spinal cord.

Organism Icon Mus musculus
Sample Icon 36 Downloadable Samples
Technology Badge IconIllumina HiSeq 2500

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Description
Translating ribosome affinity purification (TRAP) was performed on spinal cord dissections pooled from 3-4 mice 21 days post birth that were positive for the eGFP-L10A fusion ribosomal marker protein under the expression of either the Chat promoter (Tg(Chat-EGFP/Rpl10a)DW167Htz) or the Snap25 promoter (Tg(Snap25-EGFP/Rpl10a)JD362Jdd). RNA-sequencing was performed on both TRAP and pre-immunoprecipitation (PreIP) control RNA samples. Overall design: Three replicates of PreIP and TRAP for two transgenic lines.
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36
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