Analyzing differential gene expression pattern upon BRUCE knockdown in full medium and starvation conditions

BRUCE was identified as a novel positive regulator of autophagy. By analyzing changes in mRNA levels, we wanted to determine whether BRUCE regulates autopahgy on a trancscriptional level. Overall design: Examination of changes in total mRNA levels comparing control (shRenilla) and BRUCE knockdown (shBruce) cells in full medium (FM) and starvation medium (Starv)

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Ebner P, Poetsch I, Deszcz L, Hoffmann T, Zuber J, Ikeda F