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Accession IconSRP119610

Non-synchronized cell cycle transcriptomics in U2OS and HeLa cancer cells

Organism Icon Homo sapiens
Sample Icon 21 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

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Description
Sorting U2OS and HeLa cells genetically modified with the Fucci System allowed us to separate cells according to cell cycle progression followed by RNA Sequencing to characterize the oscillating transcriptome in cells without the need for chemical synchronization. Overall design: HeLa cells were sorted at three timepoints, while U2OS cells were sorted at two timepoints. Each time into three groups, categorized as "G1", "S", and "G2".
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21
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Cell line
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