github link
Accession IconSRP127547

mRNA sequencing to assess RfxCasR and matching shRNA specificity

Organism Icon Homo sapiens
Sample Icon 21 Downloadable Samples
Technology Badge IconNextSeq 500

Submitter Supplied Information

Description
Matching sets of RfxCasR and shRNAs targeting ANXA4 and B4GALNT1 plus non-targeting (NT) controls were profiled by mRNA sequencing to compare non-specific transcriptome perturbations for both shRNA and RfxCasR technologies. Overall design: Three biological replicates for 3 shRNAs and 2 RfxCasR guide RNAs plus 2 RfxCasR arrays expresssed in HEK 293FT cells
PubMed ID
Publication Title
Total Samples
21
Submitter’s Institution
No associated institution
Authors
Source Repository

Samples

Show
of 21 Total Samples
Filter
Add/Remove
Accession Code
Title
Cell line
Treatment
Subject
Processing Information
Additional Metadata
SRR6418949
shRNA NT rep 1
293ft kidney cell line
shrna nt
shrna nt, 293ft kidney cell line
NA
SRR6418954
shRNA ANXA4 rep 3
293ft kidney cell line
shrna anxa4
shrna anxa4, 293ft kidney cell line
NA
SRR6418951
shRNA NT rep 3
293ft kidney cell line
shrna nt
shrna nt, 293ft kidney cell line
NA
SRR6418952
shRNA ANXA4 rep1
293ft kidney cell line
shrna anxa4
shrna anxa4, 293ft kidney cell line
NA
SRR6418953
shRNA ANXA4 rep 2
293ft kidney cell line
shrna anxa4
shrna anxa4, 293ft kidney cell line
NA
SRR6418955
shRNA B4GALNT1 rep 1
293ft kidney cell line
shrna b4galnt1
shrna b4galnt1, 293ft kidney cell line
NA
SRR6418956
shRNA B4GALNT1 rep 2
293ft kidney cell line
shrna b4galnt1
shrna b4galnt1, 293ft kidney cell line
NA
SRR6418957
shRNA B4GALNT1 rep 3
293ft kidney cell line
shrna b4galnt1
shrna b4galnt1, 293ft kidney cell line
NA
SRR6418958
RfxCasR NT gRNA rep 1
293ft kidney cell line
rfxcasr nt grna
rfxcasr nt grna, 293ft kidney cell line
NA
SRR6418950
shRNA NT rep 2
293ft kidney cell line
shrna nt
shrna nt, 293ft kidney cell line
NA
Loading...
Some fields may be harmonized. Learn more