Histone variant H3.3-mediated chromatin remodeling is essential for paternal genome activation in mouse preimplantation embryos

H3.3-mediated paternal chromatin remodeling is essential for the development of preimplantation embryos and the activation of the paternal genome during embryogenesis. Overall design: We collected embryos of WT, mH3.3KD and H3.3-addback at 50h after ICSI (8-cell or 16-cell stage). RNA-seq results confirmed excellent knockdown of both H3.3A and H3.3B mRNAs in mH3.3KD and H3.3-addback embryos.

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Kong Q, Banaszynski LA, Geng F, Zhang X, Zhang J, Zhang H, O'Neill CL, Yan P, Liu Z, Shido K, Palermo GD, Allis CD, Rafii S, Rosenwaks Z, Wen D