NextSeq 500 paired end sequencing; GSM3131150: Sample_10 [RNA-Seq 2]; Homo sapiens; RNA-Seq

Sample_10 [RNA-Seq 2]

Maternal plasama colected longitudinally were profiled using targeted sequencing (DriverMap™) (https://www.cellecta.com) to evaluate changes with gestational age and with labor in normal pregnancy. Overall design: The study included normal pregnancies with (TIL) (n=8) and without (TNL) (n=8) spontaneous labor at term.  Half of the women in each group had 3 longitudinal samples taken from 12.1-40.3 weeks of gestation, while the other half of women had only one sample taken at term before delivery, for a total of 32 samples.  Note that Sample_26 was considered contaminated and hence data for that sample was not included in downstream analyses.

Targeted sequencing based maternal whole blood expression changes with gestational age and labor in normal pregnancy

Submitted by Gene Expression Omnibus on 09-FEB-2019

Maternal plasama colected longitudinally were profiled using targeted sequencing (DriverMapâ„¢) (https://www.cellecta.com) to evaluate changes with gestational age and with labor in normal pregnancy. Overall design: The study included normal pregnancies with (TIL) (n=8) and without (TNL) (n=8) spontaneous labor at term.  Half of the women in each group had 3 longitudinal samples taken from 12.1-40.3 weeks of gestation, while the other half of women had only one sample taken at term before delivery, for a total of 32 samples.  Note that Sample_26 was considered contaminated and hence data for that sample was not included in downstream analyses.

RNA was isolated from  PAXgene® Blood RNA collection tube (BD 762165) as described in the PAXgene® Blood miRNA Kit Handbook (December, 2015). Purified RNA was quantified by UV spectrophotometry using the DropSense96® Microplate Spectrophotometer (Trinean) and quality assessed by microfluidics using the RNA ScreenTape on the Agilent 2200 TapeStation. The DriverMap™ Human Genome-Wide Gene Expression Profiling Assay (hDM18Kv2) from Cellecta, Inc (Mountain View, CA) was used to measure the expression level of 18,559 protein coding genes by combining highly multiplexed RT-PCR amplification with Next-Generation Sequencing (NGS) quantitation. Sample processing was performed per manufacturer protocols available at https://www.cellecta.com/technology-portfolio/targeted-expression-profiling-driver-map-assay/. cDNA products amplified in the assay were analyzed on an Illumina NextSeq 500 sequencer using a NextSeq 500/550 High Output v2 kit (75 cycles).

Targeted sequencing based maternal whole blood expression changes with gestational age and labor in normal pregnancy

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