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Accession IconSRP145589

Expression analysis of PC3 cells treated with scramble AON or AON directed against MBNL1

Organism Icon Homo sapiens
Sample Icon 24 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

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Description
We transfected PC3 cells with 100nM of a scrambled antisense oligonucleotide (AON) and an AON directed against MBNL1 exon 7 (36 basepairs) in order to skip the latter. Cells were harvested at 72h post-transfection and RNAseq was performed with ribozero depletion. Overall design: For RNA-Seq library preparation we followed the Illumina TruSeq RNA Sample Preparation Kit v2 manual. At least 70 million, 75bp long paired end reads were mapped to the GRCh37/hg19 version of the human genome per replicate using STAR 2.4.2a (doi: 10.1093/bioinformatics/bts635) using the default parameters.
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24
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