refine.bio helps you build ready-to-use datasets with normalized transcriptome data from all of the world’s genetic databases.
SRP149631
Low Cell-Matrix Adhesion Reveals Two Subtypes of Human Pluripotent Stem Cells
Homo sapiens
8 Downloadable Samples
Illumina HiSeq 2500
Submitter Supplied Information
Description
The human pluripotent stem cells cultured on Low-adhesion substrates formed two morphologically different subtypes (Monolayer and Domed). The dome-like cells showed higher proliferation capacity and KLF4/5 expression than the monolayer cells. A serum response factor based regulatory double loop was proposed to explain how cell-matrix adhesion mediates the interaction between cell morphology and pluripotency genes Overall design: Compare the two type cells with RNA-seq to investigate the different expression