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Accession IconSRP149834

Exon Level Machine Learning Analyses Elucidate Novel Candidate miRNA Targets in an Avian Model of Fetal Alcohol Spectrum Disorder

Organism Icon Gallus gallus
Sample Icon 4 Downloadable Samples
Technology Badge IconIllumina Genome Analyzer IIx

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Description
An RNA-seq dataset obtained from neural fold-stage chicken (Gallus gallus, strain Special Black) embryos that were exposed to a pharmacologically-relevant alcohol concentration (52 mM for 90 min) or isotonic saline. The cranial headfolds were isolated 6 hours following the initial alcohol exposure. Following RNA isolation, cDNA synthesis, and quality assurance (20), paired-end reads (75 bp) were generated on an Illumina Genome Analyzer IIx (University of Wisconsin Biotechnology Center). Overall design: Paired end runs with 2 replicate ethanol exposed samples (pool of 23 individual neural folds) and 2 saline control samples (pool of 23 individual neural folds).
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4
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