Next Generation Sequencing Facilitates Quantitative Analysis of ischemic postconditioning (IPO) to attenuate hepatic ischemia-reperfusion injury in mice

This is the first study to investigate mRNA expression profiling in regard to hepatic I/R and IPO by next-generation RNA-Seq. Our results may provide an experimental basis for elucidating the underlying mechanism of IPO and reveal candidate biomarkers with which to assess hepatic I/R injury Overall design: liver mRNA profiles of sham, I/R and IPO mice were generated by next-generation sequencing, in triplicate, using Illumina HiSeq 4000.

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Zhang P, Ming Y, Cheng K, Niu Y, Ye Q