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Accession IconSRP174634

Single-cell RNA-seq of Mesp1+ mesoderm from ES cells

Organism Icon Mus musculus
Sample Icon 48 Downloadable Samples
Technology Badge IconIllumina HiSeq 2500

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Description
We have developed a protocol to generate hematopoietic and cardiac derivatives in vitro by Mesp1 induction in ES cells. The goal of this study is to analyze the heterogeneity of Mesp1+ mesoderm by single-cell RNA-seq Overall design: 48 Mesp1-induced single cells were captured using the Fluidigm C1 microfluidic system. RNA extraction, RT and cDNA amplication were then performed according to the manufacturer's manual.
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48
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No associated institution

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