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Accession IconSRP182916

Transcriptomic profiling of human peripheral blood-derived mast cells

Organism Icon Homo sapiens
Sample Icon 12 Downloadable Samples
Technology Badge IconNextSeq 500

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Description
Here, we characterized the transcriptome of MCs under steady state, immunoglobulin E (IgE)-sensitized and anti-IgE-treated conditions. Overall design: MCs were left untreated or sensitized overnight with myeloma-IgE (0.5 µg/ml) and treated with anti-IgE (1 µg/ml) for 2h. RNA was isolated with Trizol and RNeasy columns and RNA-seq was performed.
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12
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