Gene expression profile during senescence induced by LSG1 knockdown

In the present study, we analyze the effect of knocking down LSG1 and KRas(V12D) overexpression in MRC5 cells in the transcriptome using Ampliseq RNA sequencig. We observed that shLSG1 induced a potent senescence response that is characterized by the activation of ER-Stress and cholesterol biosynthetic pathway Overall design: MRC5 were transfected with siRNA to knockdown the small GTPase LSG1. Total mRNA was extracted and expression profiles were analyzed.

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Pantazi A, Quintanilla A, Hari P, Tarrats N, Parasyraki E, Dix FL, Patel J, Chandra T, Acosta JC, Finch AJ