The fetal ovarian grafts under the kidney capsule of adult male mice undergo a partial sex-reversal showing ectopic SOX9-positive Sertoli cell-like cells around 15-20 days post-transplantation. However, the molecular bases of such masculinization of fetal ovaries in the paternal environment were unclear.
Molecular and genetic characterization of partial masculinization in embryonic ovaries grafted into male nude mice.
Specimen part
View SamplesTo clarify the functional role of migratory liver-resident leukocytes (LRLs) in the pre-metastatic lung, we identify differentially expressed genes and address biological significance in the liver.
Hepato-entrained B220<sup>+</sup>CD11c<sup>+</sup>NK1.1<sup>+</sup> cells regulate pre-metastatic niche formation in the lung.
Specimen part, Disease, Disease stage
View SamplesTo understand the molecular mechanisms mediating Liver Resident Leukocytes (LRL) relocalization from the liver to the lungs in response to tumor progression, isolated LRLs from the liver and lungs of tumor-stimulating mice using a cell sorter. LRLs remaining in the liver displayed increased liver signature when compared to those that migrated into the lungs.
Hepato-entrained B220<sup>+</sup>CD11c<sup>+</sup>NK1.1<sup>+</sup> cells regulate pre-metastatic niche formation in the lung.
Specimen part
View SamplesExpression data from mice exposed to intermittent hypoxia and mice reared for 12 months. We used microarrays to analyze the transcriptome of hippocampus from mice exposed to intermittent hypoxia or aged mice.
Treatment of intermittent hypoxia increases phosphorylated tau in the hippocampus via biological processes common to aging.
Specimen part, Treatment
View SamplesAddition of CO2 to the inspired gas can ameliorate lung injury during high tidal volume mechanical ventilation in animal models. Although some effects of hypercapnia on physiology and cell signaling have been characterized, we hypothesized that assessment of genome-wide gene expression patterns would reveal novel pathways of protection.
α-Tocopherol transfer protein mediates protective hypercapnia in murine ventilator-induced lung injury.
Sex, Specimen part
View SamplesIdentifying novel candidate biomarker gene differentially expressed in the peripheral blood cells of patients with early stage acute myocardial infarction using microarray as a high throughput screening technology.
Novel genes detected by transcriptional profiling from whole-blood cells in patients with early onset of acute coronary syndrome.
Specimen part, Disease, Time
View SamplesStudy of the Arabidopsis circadian clock.
A hierarchical multi-oscillator network orchestrates the Arabidopsis circadian system.
Specimen part
View SamplesIn mammals, chromosomes are partitioned into megabase-sized topologically associating domains (TADs). TADs can be in either A (active) or B (inactive) subnuclear compartments, which correspond to early (E) and late (L) replicating timing (RT) domains, respectively. Here, we show that RT changes are tightly correlated with A/B compartment changes during mouse embryonic stem cell (mESC) differentiation. A/B compartments changed mostly by a “boundary shift,” frequently causing compartment switching of single TADs, which coincided with or preceded RT changes. Upon differentiation, mESCs acquired an A/B compartment organization that closely resembled EpiSCs (epiblast-derived stem cells), suggesting that accumulation of compartment boundary repositioning eventually led to naïve-to-primed pluripotency transition in A/B compartment organization. We propose that large-scale reorganization of A/B compartments, which is reflected in RT domain reorganization, represents major cell fate changes. Collectively, our data provides valuable insights into the regulatory principles of 3-dimensional (3D) genome organization during early embryonic stages. Overall design: RNA-Seq: 9 cell types, with a total of 34 individual replicates.
Single-cell DNA replication profiling identifies spatiotemporal developmental dynamics of chromosome organization.
Specimen part, Subject
View SamplesWe used an Aldefluor assay system to study the signaling pathways that regulate the frequency and maintenance of ALDH-positive cell population in MCF-7 cells as a CSC model.
Sphingosine-1-phosphate promotes expansion of cancer stem cells via S1PR3 by a ligand-independent Notch activation.
Cell line
View SamplesComparison of gene expression profile between an 96 day human retina and photoreceptors present around the same time.
Generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem cells.
Specimen part
View Samples