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GSE45747
Homo sapiens
16 Downloadable Samples
Affymetrix Human Genome U219 Array (hgu219), Illumina Genome Analyzer IIx
Description
This SuperSeries is composed of the SubSeries listed below.
Publication Title
Weight loss after gastric bypass surgery in human obesity remodels promoter methylation.
Sample Metadata Fields
Sex, Specimen part
View Samples- Homo sapiens
- 16 Downloadable Samples
- Illumina Genome Analyzer IIx, Affymetrix Human Genome U219 Array (hgu219)
Description
Profiling of gene expression in Vastus Lateralis from female patients before and after GBP surgery and from lean Control
Publication Title
Weight loss after gastric bypass surgery in human obesity remodels promoter methylation.
Sample Metadata Fields
Sex, Specimen part
View Samples- Homo sapiens
- 21 Downloadable Samples
- Affymetrix Human Gene 1.1 ST Array (hugene11st)
Description
This SuperSeries is composed of the SubSeries listed below.
Publication Title
Altered DNA methylation of glycolytic and lipogenic genes in liver from obese and type 2 diabetic patients.
Sample Metadata Fields
Sex, Specimen part, Disease stage
View Samples- Homo sapiens
- 21 Downloadable Samples
- Affymetrix Human Gene 1.1 ST Array (hugene11st)
Description
Type 2 diabetes is a complex disease associated with many underlying pathomechanisms. Epigenetic regulation of gene expression by DNA methylation has become increasingly recognized as an important component in the etiology of type 2 diabetes. We performed genome-wide methylome and transcriptome analysis in liver from severely obese patients with or without type 2 diabetes to discover aberrant pathways underlying the development of insulin resistance. We identified hypomethylation of five key genes involved in hepatic glycolysis, de novo lipogenesis and insulin resistance with concomitant increased mRNA expression and protein content. The CpG-site within the ATF-motif was hypomethylated in four of these genes in liver of non-diabetic and type 2 diabetic obese patients, suggesting epigenetic regulation of transcription by altered ATF-DNA binding. In conclusion, severely obese non-diabetic and type 2 diabetic patients have distinct alterations in the hepatic methylome and transcriptome and genes controlling glucose and lipid metabolism are hypomethylated at a regulatory site. Thus, obesity may epigenetically reprogram the liver towards increased lipid production and exacerbate the development of insulin resistance.
Publication Title
Altered DNA methylation of glycolytic and lipogenic genes in liver from obese and type 2 diabetic patients.
Sample Metadata Fields
Sex, Specimen part, Disease stage
View Samples- Mus musculus
- 6 Downloadable Samples
- Affymetrix Mouse Gene 2.0 ST Array (mogene20st)
Description
We used microarrays to detail the gene expression profile during WAT -beige transition by treatment of beta adrenergic receptor agonist .
Publication Title
Endothelial PDGF-CC regulates angiogenesis-dependent thermogenesis in beige fat.
Sample Metadata Fields
Specimen part
View Samples