Search for transcripts encoding secreted proteins whose expression are highly induced after phlebotomy.
Identification of erythroferrone as an erythroid regulator of iron metabolism.
Sex, Age, Specimen part, Time
View SamplesPurpose: To understand the molecular mechanisms underlying NPM1c-mediated tumorigenesis by comparing the transcriptome of de novo generated bulk human leukemic cells and leukemic stem cells Overall design: Human hematopoietic stem/progenitor cells (HSPC) are transduced with lentiviruses expressing a mutated form of Nucleophosmin (NPM1c). Following engraftment into immunodeficient mice, transduced HSPCs give rise to human myeloid leukemia whereas untransduced HSPCs give rise to human immune cells in the same mice. The de novo AML, with CD123+ leukemic stem cells (LSC), resembles NPM1c+ AML from patients.
Induction and Therapeutic Targeting of Human NPM1c<sup>+</sup> Myeloid Leukemia in the Presence of Autologous Immune System in Mice.
Specimen part, Subject
View SamplesThe SKOV-3 and Caov-3 ovarian cancer cell lines where seeded onto the 100mm cell culture plate for overnight. The cells were then incubated with IL-6 (30ng/ml) alone for one hour or pre-treated with siltuximab for four hours and then treated with IL-6 for one hour. Total RNA was collected from these cells using TRIzol Reagent (GIBCO Grand Island, NY) according to the manufacturers instructions. To account for and eliminate biologic noise, RNA was isolated from three distinct flasks of each cell line and pooled together. RNA quality was determined via ethidium bromide staining following agarose/formaldehyde gel electrophoresis. Total RNA was processed and hybridized to Affymetrix Genechip HG-U95Av2 arrays (Affymetrix, Santa Clara, CA) by the Gene Array Technology Center at Harvard Medical School (http://genome.med.harvard.edu). Affymetrix Gene Chip U133 Plus 2.0 in the first and most comprehensive whole human genome expression array. This array completes coverage of the whole human Genome with over 47,000 transcripts. The expression level of each mRNA is quantified by measuring its hybridization to these 23-mers in comparison to its hybridization to a one-base mismatch oligonucleotide. GeneSifter was used to analyze the microarray data (http://www.genesifter.net/web/). Fold change in expression between sensitive and resistant cell lines was evaluated using the Mann-Whitney test. A tenfold or greater change in intensity combined with a Mann-Whitney associated P value less than 0.05 was used as the criterion for inclusion in our filtered data set.
Effects of siltuximab on the IL-6-induced signaling pathway in ovarian cancer.
Specimen part, Cell line
View SamplesThis study provides a comparison of genes expressed in reconstructed cultured epidermis derived from four different donors.
Xenobiotic metabolism gene expression in the EpiDermin vitro 3D human epidermis model compared to human skin.
Sex, Specimen part
View SamplesThis SuperSeries is composed of the SubSeries listed below.
Frequent MYC coamplification and DNA hypomethylation of multiple genes on 8q in 8p11-p12-amplified breast carcinomas.
Age, Specimen part
View SamplesGenomic and expression profiling using 38K BAC array-CGH and Illumina HT-12 beadchips were performed on 97 diploid invasive breast tumors to assess the impact of gene dosage on gene expression patterns and the effect of other mechanisms on transcriptional levels. Patient stratification was performed according to axillary lymph node status (node-negative, pN0; node-positive, pN1) and overall survival (>8-year survivors; breast cancer-specific mortality within 8 years of diagnosis). Array-CGH results was validated by FISH using tumors showing HER2/neu gene amplification and expression profiling was confirmed using qPCR for 16 transcripts.
Clinical implications of gene dosage and gene expression patterns in diploid breast carcinoma.
Disease, Disease stage
View SamplesGenomic and expression profiling using 38K BAC array-CGH and Illumina HT-12 beadchips were performed on 97 diploid invasive breast tumors to assess the impact of gene dosage on gene expression patterns and the effect of other mechanisms on transcriptional levels. Patient stratification was performed according to axillary lymph node status (node-negative, pN0; node-positive, pN1) and overall survival (>8-year survivors; breast cancer-specific mortality within 8 years of diagnosis). Array-CGH results was validated by FISH using tumors showing HER2/neu gene amplification and expression profiling was confirmed using qPCR for 16 transcripts.
Clinical implications of gene dosage and gene expression patterns in diploid breast carcinoma.
Disease, Disease stage
View SamplesTranscriptomic profiling of human breast tumors.
Clinical implications of gene dosage and gene expression patterns in diploid breast carcinoma.
Age, Specimen part
View SamplesAnalysis of peripheral blood mononuclear cells (PBMCs) separated from whole blood of healthy male subjects
Effects of exercise on gene expression in human peripheral blood mononuclear cells.
No sample metadata fields
View SamplesThis SuperSeries is composed of the SubSeries listed below.
Coexpression of normally incompatible developmental pathways in retinoblastoma genesis.
Specimen part
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