Leukemia Inhibitory Factor is an important cytokine of the IL family. Recent findings suggest it has a crucial role in cancer progression
Leukemia Inhibitory Factor Promotes Aggressiveness of Chordoma.
Cell line
View SamplesInterleukin-15 (IL-15) and IL-2 possess distinct immunological functions despite both signaling through IL-2Rß and the common cytokine receptor ?-chain, ?c, We find that in the IL-15/IL-15Ra/IL-2Rß/?c quaternary complex structure, IL-15 heterodimerizes IL-2Rß and ?c identically to the IL-2/IL-2Ra/IL-2Rß/?c complex, despite differing receptor-binding chemistries. IL-15Ra dramatically increases the affinity of IL-15 for IL-2Rß, and this allostery is required for IL-15 trans-signaling versus IL-2 cis-signaling. Consistent with the identical IL-2Rß/?c dimer geometry, IL-2 and IL-15 exhibited similar signaling properties in lymphocytes, with any differences resulting from disparate receptor affinities. Thus, IL-15 and IL-2 induce similar signals, and the cytokine-specificity of IL-2Ra versus IL-15Ra determines cellular responsiveness. These results provide important new insights for specific development of IL-15- versus IL-2-based immunotherapeutics. Overall design: RNA-Seq is conducted in mouse CD8+ T cells, not treated or treated with IL2 or IL15 for indicated concentrations (1nM or 500nM) and times (4hr or 24hr).
Mechanistic and structural insight into the functional dichotomy between IL-2 and IL-15.
Specimen part, Cell line, Treatment, Subject
View SamplesThe endoplasmic reticulum (ER) is the site of secretory lipoprotein production and de novo cholesterol synthesis, yet little is known about how these activities are coordinated with each other, or with the activity of the COPII machinery, which transports ER cargo to the Golgi. The Sar1B component of this machinery is mutated in Chylomicron Retention Disorder, establishing that this Sar1 isoform secures delivery of dietary lipids into the circulation.
The endoplasmic reticulum coat protein II transport machinery coordinates cellular lipid secretion and cholesterol biosynthesis.
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