In this work, we isolated and characterized a novel cell population derived from human amniotic fluid cells (hAKPC-P), and we differentiated them into podocytes.
A novel source of cultured podocytes.
Specimen part, Cell line
View SamplesMM1S cells have been cultured under normoxic and hypoxic conditions, and gene expression profiling has been performed using the Affymetrix Human Genome U133 Plus 2.0 array.
Metabolic signature identifies novel targets for drug resistance in multiple myeloma.
Cell line
View SamplesNGS technology was used for high-throughput profiling of the transcriptome by comparing satellite cells lacking or not HDAC4. Overall design: Total RNA was isolated from control and HDAC4 KO satellite cells in growth conditions
HDAC4 regulates satellite cell proliferation and differentiation by targeting P21 and Sharp1 genes.
Age, Specimen part, Cell line, Subject
View SamplesBackground: circular RNAs are a class of endogenous RNAs with various functions in eukaryotic cells. Worthy of note, circular RNAs play a critical role in cancer. Currently, nothing is known about the role of circular RNAs in head and neck squamous cell carcinoma (HNSCC). The identification of circular RNAs in HNSCC might become useful for diagnostic and therapeutic strategies in HNSCC.
The oncogenic role of circPVT1 in head and neck squamous cell carcinoma is mediated through the mutant p53/YAP/TEAD transcription-competent complex.
Sex, Specimen part, Disease, Disease stage
View SamplesFSHD myoblasts show a suppression of ESRRA and PPARGC1A during myogenesis Overall design: FSHD Myoblasts 54-2, 54-12, 54-A5, 16A and 12A and matched controls 54-6, 54-A10, 16U and 12U were plated at 312,000 cells per 12 well plate in proliferation media and cultured for 48 hours or until 100% confluent, then induced to differentiate for 3.5 days, samples were taken at 8 time points during differentation for 54-6 and 54-12 and at confluency and terminal differentiation in the remaining lines. RNA-sequencing was performed on high quality (RIN > 8.0) DNA free RNA.
Dynamic transcriptomic analysis reveals suppression of PGC1α/ERRα drives perturbed myogenesis in facioscapulohumeral muscular dystrophy.
Sex, Subject
View SamplesThere is high need of novel diagnostic and prognostic tools for tumors of the digestive system, such as gastric cancer and cholangiocarcinoma.
MiR-204 down-regulation elicited perturbation of a gene target signature common to human cholangiocarcinoma and gastric cancer.
Specimen part
View SamplesThis SuperSeries is composed of the SubSeries listed below.
Cell-type-specific control of enhancer activity by H3K9 trimethylation.
Specimen part
View SamplesThis SuperSeries is composed of the SubSeries listed below.
Role of cell-type specific nucleosome positioning in inducible activation of mammalian promoters.
Specimen part, Treatment
View SamplesIn dendritic cells, expression of the H3K9me3 demethylase JmjD2d is upregulated by LPS stimulation. To identify genes whose induction by LPS depends on JmjD2d activity, we performed a microarray analysis of wild-type and JmjD2d-knockdown dendritic cells, before and after stimulation with LPS.
Cell-type-specific control of enhancer activity by H3K9 trimethylation.
No sample metadata fields
View SamplesWe used microarrays to asses gene expression in 3T3 fibroblasts, after knock-down of Brg1 and Brm using stable shRNA interference. Cells were treated with 5ng/ml mouse TNF-alpha to stimulate inducible gene activation.
Role of cell-type specific nucleosome positioning in inducible activation of mammalian promoters.
No sample metadata fields
View Samples