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Homo sapiens
6 Downloadable Samples
Affymetrix Human Gene 1.0 ST Array (hugene10st)
Description
During pregnancy, the myometrium remains quiescent but at term, switches to a state capable of producing a series of coordinated contractions for the delivery of the fetus. Myometrial contractions of labour signify the normal physiological end-point of pregnancy but the biochemical onset of labour may occur at or before term via a series of changes in expression of labour associated genes that are responsible for controlling the activity of the uterus during pregnancy and parturition. There is increasing evidence that components of the cAMP-signalling pathway are up-regulated in the human myometrium during pregnancy to promote the relaxation of the myometrium until term. Our aim was to determine which cAMP-associated genes are important during pregnancy and parturition by exposing myometrial cells to forskolin and performing an a gene array. We then plan to study the trend of the cAMP-associated genes at different stages of gestation and during labour.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Homo sapiens
- 37 Downloadable Samples
Illumina HiSeq 4000
Description
For quantification of RNA transcript using RT-qPCR data, normalization of the data by the internal control reference genes is often required. However, it has been demonstrated that a proper choice of reference genes is highly dependent on the tissues or cells being investigated. It has also been known that reference genes are highly specific for a particular experimental model, and validation for each situation, on an individual basis, is essential. Currently, there is a lack of data on reference genes that are suitable for normalization of RT-qPCR data in the blood circulation of pregnant women. The objective of this study is to identify reference genes in maternal blood based on the whole-transcriptome data of 19 maternal whole blood samples, sequenced on the HiSeq-4000 platform in two libraries (technical replicates) per sample.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Disease, Cell line
View Samples- Homo sapiens
- 62 Downloadable Samples
- Illumina HiSeq 4000
Description
We have designed an integrated model with clinical and genomic data to estimate recurrence risk for patients diagnosed with endometrioid endometrial adenocarcinoma
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Disease, Disease stage
View Samples- Homo sapiens
- 10 Downloadable Samples
- Illumina HiSeq 2500
Description
Endometriosis is a common gynecological condition with an unclear pathogenesis. Changes in lncRNA expression profiles may influence this disease, while relevant investigation remains insufficient. To describe the different lncRNA and mRNA expression patterns between endometriosis and a control group, eutopic and normal endometrium in the proliferative phase were analyzed using RNA sequencing.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part
View Samples- Homo sapiens
- 6 Downloadable Samples
- Affymetrix Human Transcriptome Array 2.0 (hta20)
Description
Long non-coding RNAs (lncRNA) have been shown to play crucial roles in tumorigenesis. Little is known about lncRNA RAD51-AS1 in diseases. Here, we investigated the role of RAD51-AS1 in Epithelial ovarian cancer. Silencing RAD51-AS1 inhibited proliferation through cell cycle arrest and promotion in apoptosis, both in vitro and in vivo. But the mechanism of RAD51-AS1 downstream regulation remains unclear.
Publication Title
E2F1-regulated long non-coding RNA RAD51-AS1 promotes cell cycle progression, inhibits apoptosis and predicts poor prognosis in epithelial ovarian cancer.
Sample Metadata Fields
Specimen part, Cell line, Time
View Samples- Homo sapiens
- 4 Downloadable Samples
- Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2)
Description
Analysis of ovarian cancer cell lines after knockdown of FGFRL1 using SiRNA.
Publication Title
FGFRL1 Promotes Ovarian Cancer Progression by Crosstalk with Hedgehog Signaling.
Sample Metadata Fields
Cell line
View Samples- Mus musculus
- 4 Downloadable Samples
- Affymetrix Mouse Genome 430 2.0 Array (mouse4302)
Description
nulliparous cd1 female mice were mated. Twenty four hours after detecting hte vaginal plug , the animals were laparatomized and the uterine lumen were transiently-transfected with plasmid harboring HOXA10 or control. Forty eight hours later, the uteri were morcellated in trizol and the RNA was extracted per Trizol protocol. Total RNA was then submitted for microarray analysis
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Subject
View Samples- Homo sapiens
- 6 Downloadable Samples
- Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2)
Description
We performed knockdown of circARID1A, overexpression of circARID1A and overexpression of miR-204-3p in ReNcell, independently. The 22,480 gene expression changes were examined by microarray analysis.
Publication Title
Genome-wide, integrative analysis of circular RNA dysregulation and the corresponding circular RNA-microRNA-mRNA regulatory axes in autism.
Sample Metadata Fields
Cell line
View Samples
SRP089972- Mus musculus
- 6 Downloadable Samples
- Illumina HiSeq 2500
Description
The experimental design was based on a control mouse vs. knockout mouse with Exons 6 and 7 of Rbpj gene being deleted in uterus. The samples were collected from mice uterus on postpartum day 3. The sampling region is described as nodal region where the previous pregnancy placentas were attached to the uterus. The goal of this study is to identify role of Rbpj in postpartum repair of uterine endometrium.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Cell line
View Samples- Homo sapiens
- 802 Downloadable Samples
- Affymetrix Human Genome U219 Array (hgu219)
Description
The host response in critically ill patients with sepsis, septic shock remains poorly defined. Considerable research has been conducted to accurately distinguish patients with sepsis from those with non-infectious causes of disease. Technological innovations have positioned systems biology at the forefront of biomarker discovery. Analysis of the whole-blood leukocyte transcriptome enables the assessment of thousands of molecular signals beyond simply measuring several proteins in plasma, which for use as biomarkers is important since combinations of biomarkers likely provide more diagnostic accuracy than the measurement of single ones or a few. Evidence suggests that genome-wide transcriptional profiling of blood leukocytes can assist in differentiating between infection and non-infectious causes of severe disease. Of importance, RNA biomarkers have the potential advantage that they can be measured reliably in rapid quantitative reverse transcriptase polymerase chain reaction (qRT-PCR)-based point of care tests.
Publication Title
A molecular biomarker to diagnose community-acquired pneumonia on intensive care unit admission.
Sample Metadata Fields
Sex, Age
View Samples