the mRNA expression of pig lung tissue. there are four groups: LW pigs infected with PCV2, LW pigs control, Yorkshire × Landrace (YL) pigs infected with PCV2, Yorkshire × Landrace (YL) pigs control.
No associated publication
Sex, Age, Specimen part, Disease, Disease stage, Cell line, Treatment
View SamplesIdentification of circular RNAs in zebrafish
Identification and characterization of circular RNAs in zebrafish.
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View SamplesSmall RNA seq following over expression of miR-218 in HepG2 cells; mRNA expression following perturbation of miR-218 in primary mouse motor neurons
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Sex, Specimen part
View SamplesProgranulin mutations found in frontotemporal lobar degeneration (FTLD) cases typically result in heterozygous loss-of-function. To assess the impact of PGRN deficiency on gene expression, particularly of genes associated with lipid metabolism, in the brain, we sequenced the total RNA extracted from whole brains from 7-month-old female Grn+/+, Grn+/–, and Grn–/– mice and determined the differential expression of transcripts in PRGN-deficient (Grn+/- and Grn-/-) and wild type (Grn+/+) brains.
No associated publication
Sex, Age, Specimen part, Cell line
View SamplesTranscriptome of connective tissue of swine affected and non-affected with scrotal hernia
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Sex, Age, Specimen part, Disease
View SamplesThe equine hoof is a specialized structure in which the distal skeleton is suspended within the capsule by interdigitated structures known as laminae. Inflammation of this tissue, known as laminitis, is a devastating disease that is the second leading cause of both lameness and euthanasia in the horse. Current research on the laminitic transcriptome focuses on the expression of known genes. However, as this tissue is quite unique and equine annotation is largely derived from computational predictions and gene models from other species, there are likely yet uncharacterized transcripts expressed in the laminae that may be involved in the etiology of laminitis. In order to create a novel annotation resource, we performed whole transcriptome sequencing of sagittal lamellar sections from one control and two laminitis affected horses. Assembly of 113 million 100bp reads resulted in around 75,000 transcripts. Of these, 36,000 corresponded to known annotation in NCBI's non-redundant protein database. RT-PCR of 12 selected annotations confirmed structure and expression in lamellar tissue. Transcriptome sequencing represents a powerful tool to expand on equine annotation and identify novel targets for further laminitis research.
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No sample metadata fields
View SamplesEffects of loss-of-function of AtMIKC* MADS-box genes on the mature Arabidopsis pollen transcriptome.
MADS-complexes regulate transcriptome dynamics during pollen maturation.
Age, Specimen part
View SamplesRNA-seq-based profiling of Mycobacterium marinum granulomas from adult zebrafish and matched macrophage samples.
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View SamplesAlthough it is well known that the function of MyoG cannot be compensated for other MRFs, the molecular mechanism by which MyoG controls muscle cell differentiation is still unclear. Therefore, in this study, RNA-Seq technology was applied to profile changes in gene expression in response to MyoG knock-down (MyoGkd) in primary bovine muscle satellite cells (MSCs). This study is the first RNA-Seq based gene expression analysis of MyoGkd undertaken in primary bovine MSCs.
No associated publication
Sex, Specimen part, Disease
View SamplesWT cells and mutants during growth on low phosphate levels and recovery
No associated publication
Specimen part, Disease, Cell line
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