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Arabidopsis thaliana
31 Downloadable Samples
NextSeq 500, Illumina HiSeq 2500
Description
This study was designed to understand the mechanism by which floral organ abscission mutants'' phenotypes arise.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Treatment
View Samples- Mus musculus
- 8 Downloadable Samples
- NextSeq 500
Description
Neuro-2a cells were infected with ZIKV (MOI = 0.5) for 48 h and total RNA was extracted and purified using TRI Reagent and RNeasy Mini kit (Qiagen). RNA-seq was performed at the Molecular and Genomics Core Facility of the University of Mississippi Medical Center. Differential expression analysis between mock and ZIKV infected cells was done using Tophat and cuffdiff programs from the tuxedo suite.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Disease, Cell line
View Samples- Anopheles gambiae
- 2 Downloadable Samples
- Illumina HiSeq 2000
Description
RNAseq from male testes
Publication Title
Odorant receptor-mediated sperm activation in disease vector mosquitoes.
Sample Metadata Fields
Sex, Specimen part
View Samples- Mus musculus
- 45 Downloadable Samples
- Illumina HiSeq 4000
Description
Viral and bacterial coinfections are common in nature, but infrequently studied in laboratory models of infection. We observed disease severity differences in mice infected with two of three possible respiratory viruses, depending on the order of the infection. To discover the mechanisms causing these differences, we compared gene expression responses of lung tissue at three time points following viral coinfection. Differential gene expression and immune cell counts suggest a dampening of immune responses in mice infected with rhinovirus followed by influenza A virus or pneumonia virus of mice.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line, Treatment
View Samples- Zea mays
- 28 Downloadable Samples
- Illumina HiSeq 2000
Description
RNA-sequencing was used on leaf tissue from 29 diverse maize lines to characterize differences in gene expression between lines. The main goal from this study was to relate gene expression to measured traits of interest.
Publication Title
No associated publication
Sample Metadata Fields
Age, Specimen part
View Samples
SRP160431- Mus musculus
- 12 Downloadable Samples
- Illumina HiSeq 2500
Description
C2C12 cells, as mouse-derived myoblasts, are a classic cell model for studying skeletal muscle development. We knocked down the immunoglobulin superfamily containing leucine-rich repeat (Islr) gene in C2C12 cell line and studied the effect of Islr on the proliferation and differentiation of C2C12 cells.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line
View Samples- Danio rerio
- 7 Downloadable Samples
Affymetrix Zebrafish Genome Array (zebrafish)
Description
Effect of ethanol or nicotine exposure on gene expression compared to control. Duplicate arrays from ethanol or nicotine treated animals compared with triplicate arrays from paired control animals. In total 4 treatment arrays (2 ethanol, 2 nicotine) and 3 control arrays (from control animals treated in parallel with ethanol-treated fish and nicotine-treated fish.)
Publication Title
Gene expression changes in a zebrafish model of drug dependency suggest conservation of neuro-adaptation pathways.
Sample Metadata Fields
Specimen part, Compound
View Samples- Gallus gallus
- 5 Downloadable Samples
- Illumina HiSeq 2000
Description
This project sequences the mRNA of the chorioallantoic membrane of Gallus gallus, the domestic chicken.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line
View Samples- Mus musculus
- 33 Downloadable Samples
- Affymetrix Mouse Genome 430 2.0 Array (mouse4302)
Description
We generated three kinds of genetically identical mouse reprogrammed cells: induced pluripotent stem cells (iPSCs), nuclear transfer embryonic stem cells (ntESCs) and iPSC-nt-ESCs that are established after successively reprogramming of iPSCs by nuclear transfer (NT). NtESCs show better developmental potential than iPSCs, whereas iPSC-nt-ESCs display worse developmental potential than iPSCs.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line
View Samples- Mus musculus
- 10 Downloadable Samples
- Affymetrix Mouse Gene 2.0 ST Array (mogene20st)
Description
Transient mitochondrial stress can promote beneficial physiological responses and longevity, termed "mitohormesis." To interrogate mitohormetic pathways in mammals, we generated mice in which mitochondrial superoxide dismutase 2 (SOD2) can be knocked-down in an inducible and reversible manner (iSOD2-KD). Depleting SOD2 only during embryonic development did not cause post-natal lethality, allowing us to probe adaptive responses to mitochondrial oxidant stress in adult mice. Liver from adapted mice had increased mitochondrial biogenesis and antioxidant gene expression and fewer reactive oxygen species. Gene expression analysis implicated non- canonical activation of the Nrf2 antioxidant and PPAR-PGC-1 mitochondrial signaling pathways in this response. Transient SOD2 knock-down in embryonic fibroblasts from iSOD2-KD mice also resulted in adaptive mitochondrial changes, enhanced antioxidant capacity, and resistance to a subsequent oxidant challenge. We propose that mitohormesis in response to mitochondrial oxidative stress in mice involves sustained basal activation of mitochondrial and antioxidant signaling pathways to establish a heightened antioxidant state.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part
View Samples