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Equus caballus
3 Downloadable Samples
Illumina HiSeq 2000
Description
The equine hoof is a specialized structure in which the distal skeleton is suspended within the capsule by interdigitated structures known as laminae. Inflammation of this tissue, known as laminitis, is a devastating disease that is the second leading cause of both lameness and euthanasia in the horse. Current research on the laminitic transcriptome focuses on the expression of known genes. However, as this tissue is quite unique and equine annotation is largely derived from computational predictions and gene models from other species, there are likely yet uncharacterized transcripts expressed in the laminae that may be involved in the etiology of laminitis. In order to create a novel annotation resource, we performed whole transcriptome sequencing of sagittal lamellar sections from one control and two laminitis affected horses. Assembly of 113 million 100bp reads resulted in around 75,000 transcripts. Of these, 36,000 corresponded to known annotation in NCBI's non-redundant protein database. RT-PCR of 12 selected annotations confirmed structure and expression in lamellar tissue. Transcriptome sequencing represents a powerful tool to expand on equine annotation and identify novel targets for further laminitis research.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Gallus gallus
- 20 Downloadable Samples
- Illumina HiSeq 2500
Description
Male broilers from two lines (n=10 per line) with different growth rate were raised at the same condition with free access to feed and drink. At day 6 and day 21, half samples of each broiler line were euthanized by cervical dislocation, and left ventricles were collected for RNA isolation. Gene expression in left ventricle was measured by RNA-seq and compared between different time points and chicken lines. The purpose of this study is to investigate gene expression change during broiler cardiac development and to compared gene expression between fast-growing modern broilers and slow-growing heritage broilers to find possible genes and pathways related to differential cardiac development and differential susceptibility to cardiac diseases between the two broiler lines.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Disease
View Samples- Gallus gallus
- 8 Downloadable Samples
- Illumina HiSeq 2500
Description
broilers heat stress.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part
View Samples- Sus scrofa
- 4 Downloadable Samples
- Illumina HiSeq 2000
Description
the mRNA expression of pig lung tissue. there are four groups: LW pigs infected with PCV2, LW pigs control, Yorkshire × Landrace (YL) pigs infected with PCV2, Yorkshire × Landrace (YL) pigs control.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Disease, Disease stage, Cell line, Treatment
View Samples- Danio rerio
- 1 Downloadable Sample
- IlluminaHiSeq4000
Description
Identification of circular RNAs in zebrafish
Publication Title
Identification and characterization of circular RNAs in zebrafish.
Sample Metadata Fields
No sample metadata fields
View Samples- Homo sapiens
- 5 Downloadable Samples
- IlluminaHiSeq2000
Description
RNA sequences are generally identical to the underlying DNA sequences but there are known exceptions. These RNA-DNA differences (RDDs) have been found in the nuclear genomes of human cells and in the mitochondria of plants and animals but not in human mitochondria. Here by deep sequencing of DNA and RNA of human mitochondria, we identified 3 RDD sites including an A-to-U and an A-to-G RDD at position 2617. Examination of the precursor polycistronic mitochondrial transcripts shows that the RDD formation occurs post-transcriptionally. Phylogenetic analysis shows that the ancestral allele at position 2617 was a thymine or a guanine. Thus the RDD formation recapitulates the ancestral form of 16S rRNA. Our findings show that RDD formation like other RNA processing steps is conserved across species and likely has functional significance.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples
SRP015012- Gallus gallus
- 6 Downloadable Samples
- Illumina HiSeq 2000
Description
Using HiSeq2000 to sequence white leghorn different parts (ovary, magnum, isthmus and uterus) of oviduct at 40-weeks.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Sus scrofa
- 2 Downloadable Samples
- Illumina HiSeq 2000
Description
Identification of candidate genes for drip loss
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part
View Samples- Drosophila melanogaster
- 66 Downloadable Samples
- Illumina HiSeq 2000
Description
This study crossed Drosophila melanogaster genotypes from four populations to the reference genome line. RNAseq data was then generated to study natural variation in allele specific expression.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line
View Samples- Sus scrofa
- 8 Downloadable Samples
- Illumina HiSeq 2500
Description
Transcriptome of connective tissue of swine affected and non-affected with scrotal hernia
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Disease
View Samples