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Homo sapiens
11 Downloadable Samples
Affymetrix Human Genome U133A Array (hgu133a)
Description
The experiment was designed to generate a time series for epithelial model during development. Each time point had 3 replicates. The data set contained 5 time points over 10 days. They are day0, day3, day5,day7,day10.
Publication Title
Dynamic and physical clustering of gene expression during epidermal barrier formation in differentiating keratinocytes.
Sample Metadata Fields
Age, Specimen part, Time
View Samples- Rattus norvegicus
- 1 Downloadable Sample
- Affymetrix Rat Genome U34 Array (rgu34a)
Description
Gene expression profiling in rat lumbar spinal cord following ventral root avulsion in the two inbred rat strains.
Publication Title
Genetically determined susceptibility to neurodegeneration is associated with expression of inflammatory genes.
Sample Metadata Fields
Sex, Specimen part, Time
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SRP059633- Homo sapiens
- 2 Downloadable Samples
IlluminaHiSeq2000
Description
Intratumoral heterogeneity is common in all tumor types and major barrier against effective cancer therapy. Especially, human malignant mesothelioma that is an environmental contaminant- induced disease is known to be extremely heterogeneous with regards to morphology as well as molecular phenotypes, resulting in poor response to cancer therapy. This study was performed to investigate the global gene expression profile of distinct subpopulations of MS1, a human malignant mesothelioma cell line
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Homo sapiens
- 26 Downloadable Samples
- Illumina Genome Analyzer IIx
Description
Using a next generation sequencer, the global transcriptional responses to acidosis were investigated in variable human cells
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Cell line
View Samples- Homo sapiens
- 2 Downloadable Samples
- Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2)
Description
This SuperSeries is composed of the SubSeries listed below.
Publication Title
let-7 Modulates Chromatin Configuration and Target Gene Repression through Regulation of the ARID3B Complex.
Sample Metadata Fields
Specimen part, Cell line
View Samples- Homo sapiens
- 2 Downloadable Samples
- Affymetrix Human Genome U133 Plus 2.0 Array (hgu133plus2)
Description
Expression of let-7i results in transcriptome alteractions in head and neck cancer cell line, OECM1.
Publication Title
let-7 Modulates Chromatin Configuration and Target Gene Repression through Regulation of the ARID3B Complex.
Sample Metadata Fields
Cell line
View Samples- Homo sapiens
- 24 Downloadable Samples
- NextSeq500
Description
BCL6 is a transcriptional repressor, and plays important biological roles in various types of cells of the immune system, and also plays a fundamental role in lymphomagenesis. Recent study showed that BCL6 is expressed in multiple myeloma (MM) cells in the bone marrow. However the role of BCL6 in MM has not yet been elucidated. This project is aiming to investigate the role of BCL6 induced in MM. We retrovirally induced BCL6 to a MM cell line, KMS12PE, and performed RNA sequencing analysis. Differentially expressed genes were determined by comparing with mock infected cells.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 17 Downloadable Samples
- Affymetrix Mouse Gene 1.0 ST Array (mogene10st)
Description
Bronchial asthma is associated with type 2 immune responses induced by components of adaptive as well as innate immunity. Although innate cytokines such as IL-25 have been shown to play key roles in development of airway hyperreactivity (AHR), little is known of innate molecules that regulate IL-25-mediated airway inflammation. We found that blockade of repulsive guidance molecule b (RGMb) in an experimental murine model of asthma blocked the development of AHR, a cardinal feature of asthma, and that RGMb is expressed on F4/80+CD11b+CD11cneg macrophages (RGMb+ macrophages), which accumulated in the lungs of OVA-sensitized and challenged mice, but not in nave mice. Moreover, we found that a large fraction of the RGMb+ macrophages expressed the IL-25 receptor IL-17RB and produced IL-13. IL-25 was critical for the development of AHR in our model, since mice deficient in IL-17RB did not develop AHR. Finally, treatment with anti-RGMb mAb during the challenge phase of the protocol after allergen sensitization effectively prevented the development of AHR and airway inflammation, suggesting for the first time that RGMb+ cells, including RGMb+ macrophages, play critical roles in allergen-induced asthma.
Publication Title
No associated publication
Sample Metadata Fields
Specimen part
View Samples- Homo sapiens
- 14 Downloadable Samples
- Illumina HiSeq 2000
Description
It is currently debated as to whether liver macrophage (LM) activation from an anti-inflammatory to pro-inflammatory phenotype contributes to obesity-induced metabolic diseases. Here we report that LMs do not undergo a pro-inflammatory phenotype switch in obesity-induced insulin resistance in mice and humans. Remarkably, immune response related genes remained also unchanged in fly immune cells (haemocytes) upon high fat feeding. However, unbiased transcriptomic analyses revealed that LMs produce non-inflammatory factors, such as insulin like growth factor binding protein 7 (Igfbp7), that directly regulate liver metabolism. Using a unique method to manipulate gene expression only in LMs in vivo, we discovered that IGFBP7 specifically produced by LMs binds the insulin receptor and induces lipogenesis and gluconeogenesis, two major pathways increased in metabolic diseases. This study shows that macrophages could contribute to insulin resistance independently of their inflammatory status and that targeting non-inflammatory factors produced by macrophages might represent a better strategy than anti-inflammatory drugs to tackle metabolic diseases.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Disease
View Samples- Homo sapiens
- 8 Downloadable Samples
- Illumina HiSeq 2500
Description
The goal of this study was to examine how miR-21 upregulation influences T cell responses. miR-21 was antagonized by lentiviral overexpression of anti-sense miR-21 during activation of naive CD4 T cells from healthy adults. On day 5 after activation, libraries of miR-21 high and miR-21 low cells were prepared using the Ovation Human FFPE RNA-Seq Library Systems (NuGEN) and sequenced on an Illumina HiSeq 2500.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part
View Samples