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Homo sapiens
20 Downloadable Samples
Illumina HiSeq 4000, Illumina HiSeq 3000, NextSeq 500
Description
Gene expression profile in Calu-3 cells infected with MERS-CoV or SARS-CoV
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Mus musculus
- 2 Downloadable Samples
- Illumina HiSeq 4000
Description
The bone marrow-derived macrophages in RPMI1640 containing 10% exo(-) FBS , were treated with apoptotic exosome-like vesicles (10 ug/ml) for 4hr, of which RNA expression was compared with non-treated control cells.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Cell line, Treatment
View Samples- Homo sapiens
- 7 Downloadable Samples
- IlluminaHiSeq2000
Description
Epstein-Barr virus (EBV) establishes lifelong infections in its human host. The virus is associated with a broad range of malignancies of lymphoid and epithelial origin, including Burkitt's lymphoma, post-transplant lymphoproliferative disease, nasopharyngeal carcinoma and gastric carcinoma. During the latent phase of its life cycle, EBV expresses more than 40 mature miRNAs that are highly abundant in tumor cells and may contribute to oncogenesis. Although multiple studies have assessed the relative expression profiles of EBV miRNAs in tumor cells, data linking these expression levels to functional target knockdown is mostly lacking. Therefore we set out to systematically assess the EBV miRNA expression levels in EBV+ tumour cell lines, and correlate this to their functional silencing capacity in these cells. We provide comprehensive EBV miRNA expression profiles of the EBV+ cell lines C666-1 (nasopharyngeal carcinoma), SNU-719 (gastric carcinoma), Jijoye (Burkitt's lymphoma), and AKBM (Burkitt's lymphoma) and of EBV- cells ectopically expressing the BART miRNA cluster. By deep sequencing the small RNA population and conducting miRNA-reporter experiments to assay miRNA potency, we were able to compare the expression profiles of the EBV miRNAs with their functional silencing efficacy. We observe a strong correlation between miRNA expression levels and functional miRNA activity. There is large variation in expression levels between EBV miRNAs in a given cell line, whereas the relative expression profiles are well maintained between cell lines. Furthermore, we show that miRNA arm selection bias is less pronounced for viral miRNAs than for human miRNAs. In addition to encoding the largest number of precursor miRNAs of all human herpesviruses, EBV expresses many miRNAs precursors that yield two functional miRNA strands, rather than one guide strand and a non-functional passenger strand. This reduced strand bias may increase the size of the EBV miRNA targetome.
Publication Title
No associated publication
Sample Metadata Fields
No sample metadata fields
View Samples- Pseudomonas aeruginosa
- 8 Downloadable Samples
- Illumina HiSeq 2500
Description
Comparison with antibiotic susceptible and multi-drug resistant Pseudomonas aeruginosa, and responses to antibiotic stresses in multi-drug resistant Pseudomonas aeruginosa
Publication Title
No associated publication
Sample Metadata Fields
Specimen part, Cell line
View Samples- Mus musculus
- 7 Downloadable Samples
- Illumina HiSeq 2500
Description
Malaria, caused by Plasmodium parasites is responsible for the illness of millions of individuals each year. Plasmodium sporozoites inoculated by mosquitoes migrate to the liver and infect hepatocytes prior to release of merozoites that initiate symptomatic blood-stage malaria. Parasites are thought to be restricted to hepatocytes throughout this obligate liver-stage of replication and differentiation. In contrast to this notion, we found that a subset of hepatic CD11c+ cells co-expressing F4/80, CD103, CD207 and CSF1R, acquired a substantial parasite burden during the liver-stage of malaria, but only after initial hepatocyte infection. These CD11c+ cells found in the infected liver and liver-draining lymph nodes exhibited transcriptionally and phenotypically enhanced antigen-presentation functions; and primed protective CD8 T cell responses against Plasmodium liver-stage restricted antigens. Our findings uncover a novel aspect of Plasmodium biology as well as the fundamental mechanism by which CD8 T cell responses are primed against liver-stage malaria.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line, Treatment
View Samples- Saccharomyces cerevisiae
- 24 Downloadable Samples
Affymetrix Yeast Genome 2.0 Array (yeast2)
Description
We profiled the expression levels of three single deletion mutants (ARO80, DAT1, RTG3) of the the wild type strain BY4742 50 minutes after rapamycin perturbation. Our goal is to validate regulatory networks inferred from microarray data E-MTAB-412.
Publication Title
Construction of regulatory networks using time series genotyped microarray data
Sample Metadata Fields
Time
View Samples
SRP075430- Homo sapiens
- 2 Downloadable Samples
- Illumina HiSeq 2500
Description
In order to validate our method to develop immortalized cellular models of microglia, we used RNA-seq to confirm the microglial phenotype of a representative clone (C20, a clonal population), which derived from the transformation of human primary microglia (human microglia purchased cryopreserved after purification from Sciencell, Cat. #1900). Transformation was carried out with vesicular stomatitis virus G envelop simian virus 40 large T antigen viral particles (VSVG SV40), containing the pBABE-puro SV40 LT construct (Addgene, Plasmid #13970), followed by VSVG containing the human telomerase reverse transcriptase (hTERT)-neomycin (pBABE-neo-hTERT) construct (Addgene, Plasmid #1774). Immortalized cells were selected in the presence of 2 µg/mL puromycin and 600 µg/mL neomycin. Individual clonal populations, including clone C20, were allowed to grow for approximately 4 weeks prior to further testing. In addition, we also used RNA-seq to verify the capacity of these cellsto respond to an inflammatory stimulus (TNF-a).
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part
View Samples- Mus musculus
- 36 Downloadable Samples
- Illumina HiSeq 1500
Description
The goal was to characterize gene expression profiles of HSPCs in mice during Bordetella pertussis infection. Mice were immunized with acellular or whole cell pertussis vaccines. HSPCs were isolated by flow cytometric sorting and RNA was prepared for library prep and RNA seq analysis.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Age, Specimen part, Disease, Cell line, Treatment
View Samples- Mus musculus
- 32 Downloadable Samples
- Illumina HiSeq 1500
Description
Total transcriptome analysis of naive or vaccinated murine lungs, post-challenge with Bordatella pertussis. Mice were immunized with PBS, whole cell pertussis vaccine, acellular pertussis vaccine, and RTX (adenylate cyclase toxoid) vaccine. The mice were then infected with B. pertussis and at 1 or 6/9 days post-challenge total lung RNA was purified for RNA-seq analysis.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Disease, Cell line, Treatment
View Samples- Homo sapiens
- 24 Downloadable Samples
- Illumina HiSeq 2500
Description
Infection of mammalian cells with vesicular stomatitis virus (VSV) results in the inhibition of cellular gene expression. This host shut-off is achieved through viral mediated inhibition of cellular gene expression at multiple levels including transcription, mRNP nuclear-cytoplasmic export, and translation. To interrogate the effects of VSV infection on translation, we infected HeLa cells at MOI 10 for 2 or 6 hours and performed polysome profiling and deep sequenced total cytoplasmic mRNA as well as monosome- and polysome-associated mRNAs. Our data support a model where viral mRNA abundance contributes to host shut-off by dominating the pool of cytoplasmic mRNA.
Publication Title
No associated publication
Sample Metadata Fields
Sex, Specimen part, Cell line, Treatment, Time
View Samples