Characterization of circular RNAs in Ovarian cancer
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View SamplesComparison of hippocampal gene expression between normal young animals and older animals with good spatial memory and older animals with poor spatial memory
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View SamplesObjective: Resident macrophages play an important role in atheromatous plaque rupture. The macrophage gene expression signature associated with plaque rupture is incompletely defined due to the complex cellular heterogeneity in the plaque. We aimed to characterise differential gene expression in resident plaque macrophages from ruptured and stable human atheromatous lesions. A cell-specific approach has the potential to address the question of gene expression differences between particular cell types in stable and unstable plaques with greater precision than approaches based on the study of whole plaques. Using laser micro-dissection, we isolated total RNA from macrophage-rich regions of stable and ruptured human atheromatous plaques derived from carotid endarterectomy samples which were comprehensively characterized using clinical, radiological and histological criteria, and carried out genome-wide gene expression profiling using microarrays.
Increased expression of fatty acid binding protein 4 and leptin in resident macrophages characterises atherosclerotic plaque rupture.
Sex, Age, Specimen part
View SamplesTranscriptomic analysis of BnLATE-regulated pathways to investigate the genome-wide effects of BnLATE overexpression on transcription
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Specimen part
View Samples1. To identify lncRNA regulating colorectal tumorigenesis, we performed RNA-seq analysis of cells with high and low tumorigenicity 2. To study the role of UPAT in colorectal cancer cells, we investigated the gene expression profiles of HCT116 cells in which UPAT expression had been suppressed by siRNA.
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Cell line
View SamplesThis study is aimed to compare the gene expression between wild type and 35S:6MYC-HARP1 in response to wounding treatment.
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Age, Specimen part, Treatment
View SamplesTransposons in maize may be involved in the formation of circRNAs and further modulate phenotypic variation. To test our hypothesis, we performed circRNA-Seq(RNase R treated) on B73 seedlings(third leaves of V3 stage), and uncovered 1,572 high-confidence maize circRNAs, which show distinct genomic features compared to linear transcripts. Comprehensive analyses showed that LINE1-like elements (LLE) and their reverse complementary pairs (RCPLLEs) are significantly enriched in the flanking regions of circRNAs.
Circular RNAs mediated by transposons are associated with transcriptomic and phenotypic variation in maize.
Specimen part, Disease
View SamplesDuring an incompatible or compatible interaction between rice (Oryza sativa) and the Asian rice gall midge (Orseolia oryzae), a lot of genetic reprogamming occurs in the plant host
Metabolic and transcriptomic changes induced in host during hypersensitive response mediated resistance in rice against the Asian rice gall midge.
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View SamplesIllumina/Solexa sequencing of CG3985 mutant and wildtype testes transcriptome (0-1 day males)
A young Drosophila duplicate gene plays essential roles in spermatogenesis by regulating several Y-linked male fertility genes.
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View SamplesThe placenta has shown morphological and functional adaption in Meishan and Yorkshire pig breeds during late gestation. While the vast difference of uterine capacity and conceptus genotype between Western and Chinese pig breeds affects the pattern of placental development. Whether the placenta within Chinese pig breed also has similar adaptive changes is still unknown. So, we measured the weight and area of 80 Chinese indigenous Diannan small-ear pig placentas and further selected 6 placentas from extremely high and low litter size groups (HL and LL) for deep RNA-sequencing to detect the molecular basis relate to the observed placental phenotype difference.
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Sex, Specimen part
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