The goal of this project is to detect the effect of visfatin on the iimmune related genes using Digital Gene Expression
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Sex, Specimen part, Cell line
View SamplesChick thymus is the critical site for T cell development and can be damaged by lipopolysaccharide (LPS) derived from Salmonella typhimurium, one of the most deleterious food-borne pathogens. However, the mechanisms remain unclear. Here we reported the first time-series transcriptome research of chick thymus after Salmonella LPS treatment. Overall design: 12 dUTP libraries of thymus samples of newly hatched male chicks were sequenced at 0, 12, 36 and 72 h post LPS treatment with 3 replications at each time point.
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Sex, Specimen part, Disease stage, Treatment
View SamplesTo understand the structural interaction of Drosophila melanogaster Rhino-Deadlock complex and to understand how various point mutation in the interacting domains affect piRNA biogenesis in vivo.
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Sex, Specimen part, Cell line
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Sex, Specimen part, Treatment
View SamplesTotal RNA of thymus from mouse different development stages were isolated and deeply sequenced by SAPAS to investigated the global gene regualtion events during thymus genesis and maturation.
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View SamplesRectal cancer stage II carcinoma and adjacent normal tissue transcriptome
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View SamplesMaize exhibits levels of structural variation (SV) of non-repeat sequences that are unprecedented among higher eukaryotes. This SV includes hundreds of copy number variants (CNVs) and thousands of presence/absence variants (PAVs). Many of the PAVs contain intact, expressed, single-copy genes that are present in one haplotype but absent from another. The goal of this project is to test the hypothesis that differences in gene copy number (both gains and losses) contribute to the extraordinary phenotypic diversity and plasticity of maize. Maize is a good model for these studies because it exhibits a rapid decay of linkage disequilibrium (LD) and because a draft genome sequence of the B73 inbred and mapping populations are available. As a first step, the "Zeanome", a near-complete set of genes present in B73, other maize lines and the wild ancestor of maize (teosinte), is being defined using transcriptomic data.
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View SamplesNo description.
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Cell line
View SamplesRNA-seq transcriptome profiles of genetically fate-mapped serotonin neurons, manually sorted from multiple anatomic domains, at both population and single cell resolution.
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Sex, Specimen part, Cell line
View SamplesHeterosis which is the improved vigor of F1-hybrids compared to their parents is widely exploited in maize (Zea mays L.) breeding to produce elite hybrids of superior yield. The transcriptomes of the maize inbred lines B73 and Mo17 and their reciprocal hybrid offspring were surveyed in the meristematic zone, the elongation zone, cortex and stele tissues of primary roots, prior to the developmental manifestation of heterosis. Single parent expression (SPE) is consistent with the dominance model for heterosis in that it denotes genes that are expressed in only one parent but in both reciprocal hybrids. In primary root tissues, between 1,027 (elongation zone) and 1,206 (stele) SPE patterns were observed. As a consequence, hybrids displayed in each tissue >400 active genes more than either parent. Analysis of tissue-specific SPE dynamics revealed that 1,233 of 2,233 SPE genes displayed SPE in all tissues in which they were expressed while 1,000 SPE genes displayed in at least one tissue a non-SPE pattern. In addition, 64% (17,351/ 27,164) of all expressed genes were assigned to the two subgenomes which are the result of an ancient genome duplication. By contrast, only between 18 and 25% of the SPE genes were assigned to a subgenome suggesting that a disproportionate number of SPE genes are evolutionary young and emerged after genome duplication. We hypothesize that this phenomenon is associated with human selection of favorable maize genotypes which might primarily affect younger genes rather than genes whose functions have been conserved for millions of years.
Nonsyntenic genes drive highly dynamic complementation of gene expression in maize hybrids.
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