This SuperSeries is composed of the SubSeries listed below.
Ibf1 and Ibf2 are novel CP190-interacting proteins required for insulator function.
Disease, Cell line
View SamplesNeural crest cells are a transient embryonic population, hence are neither present after bith and nor are they readily accesible for analysis. Therefore, little is known about the genetic networks that regulate NC especification, delamitation and migration from the dorsal neural tube to their final destination along the embryo.
Delamination of neural crest cells requires transient and reversible Wnt inhibition mediated by Dact1/2.
Specimen part
View SamplesGene expression in S2 cells after CG9740 or CP190 RNAi
Ibf1 and Ibf2 are novel CP190-interacting proteins required for insulator function.
Cell line
View SamplesPolycomb group proteins (PcG) are well known by their function in the regulation of developmental processes. PcG mediated regulation of genetic programs required for proper development are triggered by EZH2 H3K27 methyltransferase activity. EZH1 can partially substitute EZH2 activity. However, unlike EZH2, EZH1 is presence in differentiated and adult tissues suggesting additional biological functions. Here we show that EZH2 is predominantly expressed in neural stem cells being essential for neural stem cells self renewal and homeostasis. There, it controls the transcriptional state of cell cycle regulators, such as CIP1. But it is also necessary to regulate genes involved in surveillance and neuroepithelial polarity. In contrast, EZH1 expression is more abundant in differentiated cells within the spinal cord and its downregulation unables neural stem cells to differentiate. All together our data reveal a complementary but non-redundant role of EZH2 and EZH1 in neurogenesis.
EZH2 regulates neuroepithelium structure and neuroblast proliferation by repressing p21.
Specimen part
View SamplesWe used microarrays to investigate gene expression changes in olfactory bulb and hippocampus brain regions under RasGRF2 absence.
No associated publication
Specimen part
View SamplesThe aim of this study consists in detecting genes regulated by N-myc in the murine cochlea
Otx2 is a target of N-myc and acts as a suppressor of sensory development in the mammalian cochlea.
No sample metadata fields
View SamplesThe aim of this study consists in detecting genes regulated by Meis2 in the murine cochlea
Meis2 Is Required for Inner Ear Formation and Proper Morphogenesis of the Cochlea.
Specimen part
View SamplesWe used Affymetrix microarrays to investigate gene expression changes in PBMCs isolated from male patients ongoing secondary prevention of CVD to determine significant modulatory effects that may have been induced by the intake of an initial dose of 8 mg of resveratrol-enriched grape extract for 6 months and then, 16 mg for a further 6 months.
One-year supplementation with a grape extract containing resveratrol modulates inflammatory-related microRNAs and cytokines expression in peripheral blood mononuclear cells of type 2 diabetes and hypertensive patients with coronary artery disease.
Sex, Specimen part, Time
View SamplesHistone deacetylase inhibitors (HDACis) have been shown to potentiate hippocampal-dependent memory and synaptic plasticity and to ameliorate cognitive deficits and degeneration in animal models for different neuropsychiatric conditions. However, the impact of these drugs on hippocampal histone acetylation and gene expression profiles at the genomic level, and the molecular mechanisms that underlie their specificity and beneficial effects in neural tissue, remains obscure. Here, we mapped four relevant histone marks (H3K4me3, AcH3K9,14, AcH4K12 and pan-AcH2B) in hippocampal chromatin and investigated at the whole-genome level the impact of HDAC inhibition on acetylation profiles and basal and activity-driven gene expression. HDAC inhibition caused a dramatic histone hyperacetylation that was largely restricted to active loci pre-marked with H3K4me3 and AcH3K9,14. In addition, the comparison of Chromatin immunoprecipitation sequencing and gene expression profiles indicated that Trichostatin A-induced histone hyperacetylation, like histone hypoacetylation induced by histone acetyltransferase deficiency, had a modest impact on hippocampal gene expression and did not affect the transient transcriptional response to novelty exposure. However, HDAC inhibition caused the rapid induction of a homeostatic gene program related to chromatin deacetylation. These results illuminate both the relationship between hippocampal gene expression and histone acetylation and the mechanism of action of these important neuropsychiatric drugs.
Genomic targets, and histone acetylation and gene expression profiling of neural HDAC inhibition.
Specimen part
View SamplesWe performed a transcriptomic analysis of Pi starvation responses in Arabidopsis thaliana (Columbia-0) wild type plants under phosphate starvation stress and in plants with altered PHR1(-like) activity, comparing mutants of phr1 and phr1-phl1 grown in phosphate-lacking medium. Results show the central role of PHR1 and functionally redundant members of its family in the control of transcriptional responses to Pi starvation.
A central regulatory system largely controls transcriptional activation and repression responses to phosphate starvation in Arabidopsis.
Specimen part
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