Th17 cells are enriched by sorting FR4-CD4+ T cells from SKG mice. A large number of Th17 cells also develop spontaneously when CD4+ T cells from IFN-g-deficient (IFN-g-/-) BALB/c mice are transferred to T cell-deficient RAG2-deficient (RAG2-/-) mice and subjected to homeostatic proliferation, whereas they fail to develop in similar transfer of IL-6-deficient (IL-6-/-) CD4+ T cells to IL-6-/- RAG2-/- mice.
Preferential recruitment of CCR6-expressing Th17 cells to inflamed joints via CCL20 in rheumatoid arthritis and its animal model.
Sex, Age, Specimen part
View SamplesComparison of gene expressions among osteogenic differentiated cells with retinoic acid, those without retinoic acid and cells before induction
No associated publication
Specimen part, Treatment
View SamplesRegnase-1 and Roquin are RNA binding proteins essential for degradation of inflammatory mRNAs and the maintenance of immune homeostasis. Recent researches have showed that Regnase-1 and Roquin target overlapping sets of mRNAs with common stem-loop structures but localize in different structures within the cell, controlling immune-related RNAs in distinct spatiotemporal processes. Regnase-1 and Roquin are expressed in T cells, and mutations of both Regnase-1 and Roquin in T cells leads to massive lymphocyte activation. Furthermore, mutation of both Regnase-1 and Roquin leads to a huge increase in the Th1, but not Th2 or Th17 population in spleens compared to T cells with either a single Regnase-1 or Roquin deficiency. To investigate Regnase-1- and Roquin-regulated genes, transcriptome analysis was conducted using CD4 T cells lacking Regnase-1 and Roquin.
No associated publication
No sample metadata fields
View SamplesTo identify transcriptio factors responsible for CXCL13 production by human CD4+ T cells, we differentiated CXCL13-producing CD4+ T cells in vitro under TGF--positive inflammatory conditions and conducted transcriptome analysis.
Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments.
Specimen part
View SamplesGene expression in response to inflammatory stimuli is controlled by both transcriptional and posttranscriptional mechanisms in immune cells. Posttranscriptional regulation that modifies mRNA stability and translation provides rapid and flexible control of gene expression and control of mRNAs stability is important for coordinating the initiation and resolution of inflammation. However, the posttranscriptional mechanisms in innate immunity remain to be clarified. This study is aiming to investigate the posttranscriptional mechanisms in innate immunity based on the roles of RNA binding proteins and RNase we identified.
No associated publication
Specimen part
View SamplesDuodenums were isolated from Regnase-1-deficient mice and their controls. Tissues were immediately homogenized in TRIzol (Life Technologies). Total RNA was isolated according to the manufacture''s protocol.
No associated publication
Age, Specimen part, Cell line
View SamplesSensorineural hearing loss (SHL) is a relatively common disease, and studies have suggested viral infection as a major cause. In the inner ear, the blood-labyrinthine barrier prevents access of the peripheral immune system; therefore, the immune system remains poorly understood. Here we found that cochlear accessory supporting cells (SCs), which are anchored by tight junctions, are organized tissue-resident macrophages. Virus-infected supporting cells change into activated macrophages and protect audiosensory receptor hair cells (HCs) against virus infection by producing interferon (IFN)-/. Moreover, we also observed bacterial phagocytosis by SCs. However, tumour necrosis factor-related apoptosis-inducing ligand (Trail), produced by virus-infected SCs, induced sensory hair loss and HC death by necroptosis. Notably, corticosteroid, the only effective drug for SHL, inhibited the virus-induced macrophage change of SCs. These results revealed an inner ear immune system, and suggest a possible mechanism for virus-induced SHL.
No associated publication
Specimen part, Treatment
View SamplesMesenchymal stem cells (MSCs) are an essential component of the bone marrow (BM) microenvironment and have shown to support cancer evolution in multiple myeloma (MM). Despite the increasing evidence that MM MSCs differ from their healthy counterparts, little knowledge exists as to whether MSCs independently influence disease outcome. The aim of the present study was to determine the importance of MSCs in disease progression and outcome in MM.
The Pattern of Mesenchymal Stem Cell Expression Is an Independent Marker of Outcome in Multiple Myeloma.
Specimen part, Disease, Subject
View SamplesObesity has considerable effects on morbidity and mortality, and the prevalence of obesity has been increasing rapidly worldwide during the past two decades. Even if obesity affects the entire individual, adipose tissue plays a central role in the development of obesity. Expression profiling of adipose tissue may give insights into the mechanisms contributing to obesity and obesity-related disorders.
Adipose tissue resting energy expenditure and expression of genes involved in mitochondrial function are higher in women than in men.
Sex, Specimen part
View SamplesPurpose: Because dexamethasone remains a key component of myeloma therapy, we wished to examine the correlation of baseline and relapse expression levels of the glucocorticoid receptor gene NR3C1 with other clinical features. Experimental Design: We investigated the clinical impact of gene expression profiling (GEP)derived expression levels of NR3C1 in 351 patients with GEP data available at baseline and in 130 with data available at relapse, among 668 subjects accrued to Total Therapy 2 (TT2).
Thalidomide in total therapy 2 overcomes inferior prognosis of myeloma with low expression of the glucocorticoid receptor gene NR3C1.
Disease, Treatment
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