Investigation of differentially expressed genes in circZbtb20 or Nr4a1 deficient ILC3s
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View SamplesInvestigation of differentially expressed genes in Ccp6-depleted D3 ESCs
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View SamplesInvestigation of differentially expressed genes in Ccp2-deficient CHILPs
IL-7Rα glutamylation and activation of transcription factor Sall3 promote group 3 ILC development.
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View SamplesInvestigation of differentially expressed genes in circKcnt2-deficient ILC3s
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View SamplesIn this study, we aimed to identify potential lncRNA deregulations associated with breast cancer malignancy instigated by MSCs-MCF7 co-culture. We profiled expression changes of lncRNAs in MCF-7 cells during EMT induced by coculture with hAD-MSCs, and found an intergenic lncRNA with proviouly unknown function (KB-1732A1.1, we termed it LincK), which was significantly elevated. Depletion of LincK decreased the growth, migration, invasion, and EMT in breast cancer cells, while overexpression of LincK exerted the opposite effects. Moreover, knockdown of LincK repressed tumorigenesis, and ectopic expression of LincK promoted tumor growth in MCF-7 xenograft model. LincKs can act as a sponge of mirR-200b, which inhibits its function in proliferation and metastasis. Thus we reported, for the rst time, the role of LincK in control of EMT and proliferation in breast cancer.
LincK contributes to breast tumorigenesis by promoting proliferation and epithelial-to-mesenchymal transition.
Cell line
View SamplesMTDH was identified to be a functional gene in cancer progression. We overexpressed the gene in MCF7 cells and explored its functions in cancer stem cell maintainance.
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Sex, Specimen part, Disease, Cell line
View SamplesMolecular genetic analyses support important roles for the AtGATA2 gene in brassinolide (BR) and light regulation of plant development. The overexpression line 6-9 of AtGATA2 suppresses the etiolated phenotype of Col-0 grown in the dark.
Integration of light- and brassinosteroid-signaling pathways by a GATA transcription factor in Arabidopsis.
Specimen part
View Samples30 collars were taken from wild type plants or antiOsLIC transgenic plants respectively. One collar from one plant only. The leaves are just sprout 2-3 cm (about 1-2 days) from the stem. For measuring the genes expression level, Wild type plants were taken as control. The developing collar from both line2 of OsLIC antisense transgenic plants and wild type were harvested at the heading stage. The position of the collar was about 1cm above the last developed collar. Total RNA was extracted from the collars using TRIzol regeant (Invitrogen, P/N 15596-018, USA) and purified by using Qiagen RNeasy columns (QIAGEN, Cat. NO. 74104). All the processes for cDNA and cRNA synthesis, cRNA fragmentation, hybridization, washing and staining, and scanning, were conducted according to the GeneChip Standard Protocol (Eukaryotic Target Preparation, Affymetrix). Poly-A RNA Control Kit and the One-Cycle cDNA Synthesis kit were used in this experiment as described in the website: http://www.affymetrix.com/products/arrays/specific/rice.affx.
OsLIC, a Novel CCCH-Type Zinc Finger Protein with Transcription Activation, Mediates Rice Architecture via Brassinosteroids Signaling.
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View SamplesThree independent sets of purified RNA from body cells and great vessel cells of zebrafish embryos were prepared and labelled. Samples were then hybridized onto a Zebrafish AffymetrixGeneChip.
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Age, Specimen part
View SamplesThe angle of rice leaf inclination is an important agronomic trait and closely related to the yield and architecture of crops. Through genetic screening, a rice gain-of-function mutant leaf inclination1, lc1, was identified . Phenotypic analysis confirmed the exaggerated leaf angels of lc1 due to the stimulated cell elongation at the collar.In this series, we compare the transcriptome of zhonghua11 and lc1 collar.
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Specimen part
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